Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice

Johannes Attems*, Arne Ittner, Kurt Jellinger, Roger M. Nitsch, Magdalena Maj, Ludwig Wagner, Jürgen Götz, Mathias Heikenwalder

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Neuropathological features in Alzheimer's Disease (AD) include the presence of hyperphosphorylated forms of the microtubule-associated tau protein (tau) in hippocampal neurones. Numerous studies indicate a neuroprotective effect of calcium-binding proteins (Ca2+ binding proteins) in neurodegenerative diseases (e.g., AD). Secretagogin is a newly described Ca2+ binding protein that is produced by pyramidal neurones of the human hippocampus. Recently, secretagogin expressing hippocampal neurones were demonstrated to resist tau-induced pathology in AD in contrast to the majority of neighbouring neurones. This suggested a neuroprotective effect of secretagogin in hippocampal neurones. Here, we investigated secretagogin expression in wild type (wt) mice as well as in hemizygous and homozygous P301L tau transgenic (tg) mice, which show pronounced and widespread tau pathology in hippocampal neurones. Secretagogin expression was analyzed at the immunohistochemical and biochemical levels in brains of age-matched wt and hemi- and homozygous tau tg mice. In wt mice hippocampal secretagogin-immunoreactive neurones were invariably detected, while immunoreactivity was much lower (P < 0.001) in tau tg mice. Of note, hippocampal secretagogin immunoreactivity was absent in 62.5% of homozygous tau tg mice. In line with this finding, Western blot analysis demonstrated a significant reduction in protein expression levels of secretagogin in homozygous tau tg compared to wt mice. Our results suggest that increased levels of tau negatively influence secretagogin expression in the hippocampus of tau tg mice.

Original languageEnglish
Pages (from-to)737-745
Number of pages9
JournalJournal of Neural Transmission
Volume118
Issue number5
DOIs
Publication statusPublished - 1 May 2011
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Calcium-binding protein
  • P301L tau transgenic mouse
  • Secretagogin
  • Tau pathology

Fingerprint Dive into the research topics of 'Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice'. Together they form a unique fingerprint.

  • Cite this

    Attems, J., Ittner, A., Jellinger, K., Nitsch, R. M., Maj, M., Wagner, L., ... Heikenwalder, M. (2011). Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice. Journal of Neural Transmission, 118(5), 737-745. https://doi.org/10.1007/s00702-011-0626-5