TY - JOUR
T1 - Regional changes in cardiac and stellate ganglion norepinephrine transporter in DOCA-salt hypertension
AU - Wehrwein, Erica A.
AU - Novotny, Martin
AU - Swain, Greg M.
AU - Parker, Lindsay M.
AU - Esfahanian, Mohammad
AU - Spitsbergen, John M.
AU - Habecker, Beth A.
AU - Kreulen, David L.
PY - 2013
Y1 - 2013
N2 - Uptake of norepinephrine via the neuronal norepinephrine transporter is reduced in the heart during deoxycorticosterone (DOCA)-salt hypertension. We hypothesized that this was due to reduced norepinephrine transporter mRNA and/or protein expression in the stellate ganglia and heart. After 4. weeks of DOCA-salt treatment there was no change in norepinephrine transporter mRNA in either the right or the left stellate ganglia from hypertensive rats (n = 5-7, p > 0.05). Norepinephrine transporter immunoreactivity in the left stellate ganglion was significantly increased (n = 4, p. < 0.05) while the right stellate ganglion was unchanged (n = 4, p > 0.05). Whole heart norepinephrine content was significantly reduced in DOCA rats consistent with reduced uptake function; however, when norepinephrine was assessed by chamber, a significant decrease was noted only in the right atrium and right ventricle (n = 6, p < 0.05). Cardiac norepinephrine transport binding by chamber revealed that it was only reduced in the left atrium (n = 5-7, p > 0.05). Therefore, 1) contrary to our hypothesis reduced reuptake in the hypertensive heart is not exclusively due to an overall reduction in norepinephrine transporter mRNA or protein in the stellate ganglion or heart, and 2) norepinephrine transporter regulation occurs regionally in the heart and stellate ganglion in the hypertensive rat heart.
AB - Uptake of norepinephrine via the neuronal norepinephrine transporter is reduced in the heart during deoxycorticosterone (DOCA)-salt hypertension. We hypothesized that this was due to reduced norepinephrine transporter mRNA and/or protein expression in the stellate ganglia and heart. After 4. weeks of DOCA-salt treatment there was no change in norepinephrine transporter mRNA in either the right or the left stellate ganglia from hypertensive rats (n = 5-7, p > 0.05). Norepinephrine transporter immunoreactivity in the left stellate ganglion was significantly increased (n = 4, p. < 0.05) while the right stellate ganglion was unchanged (n = 4, p > 0.05). Whole heart norepinephrine content was significantly reduced in DOCA rats consistent with reduced uptake function; however, when norepinephrine was assessed by chamber, a significant decrease was noted only in the right atrium and right ventricle (n = 6, p < 0.05). Cardiac norepinephrine transport binding by chamber revealed that it was only reduced in the left atrium (n = 5-7, p > 0.05). Therefore, 1) contrary to our hypothesis reduced reuptake in the hypertensive heart is not exclusively due to an overall reduction in norepinephrine transporter mRNA or protein in the stellate ganglion or heart, and 2) norepinephrine transporter regulation occurs regionally in the heart and stellate ganglion in the hypertensive rat heart.
UR - http://www.scopus.com/inward/record.url?scp=84893907141&partnerID=8YFLogxK
U2 - 10.1016/j.autneu.2013.08.070
DO - 10.1016/j.autneu.2013.08.070
M3 - Article
C2 - 24075956
AN - SCOPUS:84893907141
SN - 1566-0702
VL - 179
SP - 99
EP - 107
JO - Autonomic Neuroscience: Basic and Clinical
JF - Autonomic Neuroscience: Basic and Clinical
IS - 1-2
ER -