TY - JOUR
T1 - Regular care and maintenance of a Zebrafish (Danio rerio) laboratory
T2 - an introduction
AU - Avdesh, Avdesh
AU - Chen, Mengqi
AU - Martin-Iverson, Mathew T.
AU - Mondal, Alinda
AU - Ong, Daniel
AU - Rainey-Smith, Stephanie
AU - Taddei, Kevin
AU - Lardelli, Michael
AU - Groth, David M.
AU - Verdile, Giuseppe
AU - Martins, Ralph N.
N1 - Copyright the Author(s) 2012. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2012
Y1 - 2012
N2 - This protocol describes regular care and maintenance of a zebrafish laboratory. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. The advantages of zebrafish in comparison to other common vertebrate models include high fecundity, low maintenance cost, transparent embryos, and rapid development. Due to the spur of interest in zebrafish research, the need to establish and maintain a productive zebrafish housing facility is also increasing. Although literature is available for the maintenance of a zebrafish laboratory, a concise video protocol is lacking. This video illustrates the protocol for regular housing, feeding, breeding and raising of zebrafish larvae. This process will help researchers to understand the natural behaviour and optimal conditions of zebrafish husbandry and hence troubleshoot experimental issues that originate from the fish husbandry conditions. This protocol will be of immense help to researchers planning to establish a zebrafish laboratory, and also to graduate students who are intending to use zebrafish as an animal model.
AB - This protocol describes regular care and maintenance of a zebrafish laboratory. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. The advantages of zebrafish in comparison to other common vertebrate models include high fecundity, low maintenance cost, transparent embryos, and rapid development. Due to the spur of interest in zebrafish research, the need to establish and maintain a productive zebrafish housing facility is also increasing. Although literature is available for the maintenance of a zebrafish laboratory, a concise video protocol is lacking. This video illustrates the protocol for regular housing, feeding, breeding and raising of zebrafish larvae. This process will help researchers to understand the natural behaviour and optimal conditions of zebrafish husbandry and hence troubleshoot experimental issues that originate from the fish husbandry conditions. This protocol will be of immense help to researchers planning to establish a zebrafish laboratory, and also to graduate students who are intending to use zebrafish as an animal model.
UR - http://www.scopus.com/inward/record.url?scp=84870051173&partnerID=8YFLogxK
U2 - 10.3791/4196
DO - 10.3791/4196
M3 - Article
C2 - 23183629
AN - SCOPUS:84870051173
SN - 1940-087X
SP - 1
EP - 8
JO - Journal of Visualized Experiments: JoVE
JF - Journal of Visualized Experiments: JoVE
IS - 69
M1 - e4196
ER -