Regulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2

Jan Hentie Swiegers, Isak S. Pretorius, Florian F. Bauer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In Saccharomyces cerevisiae the Ras/cAMP/PKA signalling pathway controls multiple metabolic pathways, and alterations in the intracellular concentrations of cAMP through modification of signalling pathway factors can be lethal or result in severe growth defects. In this work, the important role of Ras2p in metabolic regulation during growth on the non-fermentable carbon source glycerol is further investigated. The data show that the overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Δ. The overexpression results in enhanced proliferation and biomass yield when cells are grown on glycerol as sole carbon source, and increases citrate synthase activity and intracellular citrate concentration. Interestingly, the suppression of cit2Δ and the enhanced proliferation and biomass yield are only observed when RAS2 is overexpressed and not in strains containing the constitutively active allele RAS2 val19. However, both RAS2 and RAS2 val19upregulated citrate synthase activity. We propose that the RAS2 overexpression results in a combination of general upregulation of respiratory growth capacity and an increase in mitochondrial citrate/ citrate synthases, which together, complement the metabolic requirements of the cit2Δ mutant. The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source.

LanguageEnglish
Pages161-171
Number of pages11
JournalCurrent Genetics
Volume50
Issue number3
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Fingerprint

Citrate (si)-Synthase
Growth
Carbon
Citric Acid
Biomass
Glycerol
Metabolic Networks and Pathways
Saccharomyces cerevisiae
Up-Regulation
Alleles
glyoxylic acid

Cite this

@article{878607140c504e7a96ef6b58346d5bea,
title = "Regulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2",
abstract = "In Saccharomyces cerevisiae the Ras/cAMP/PKA signalling pathway controls multiple metabolic pathways, and alterations in the intracellular concentrations of cAMP through modification of signalling pathway factors can be lethal or result in severe growth defects. In this work, the important role of Ras2p in metabolic regulation during growth on the non-fermentable carbon source glycerol is further investigated. The data show that the overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Δ. The overexpression results in enhanced proliferation and biomass yield when cells are grown on glycerol as sole carbon source, and increases citrate synthase activity and intracellular citrate concentration. Interestingly, the suppression of cit2Δ and the enhanced proliferation and biomass yield are only observed when RAS2 is overexpressed and not in strains containing the constitutively active allele RAS2 val19. However, both RAS2 and RAS2 val19upregulated citrate synthase activity. We propose that the RAS2 overexpression results in a combination of general upregulation of respiratory growth capacity and an increase in mitochondrial citrate/ citrate synthases, which together, complement the metabolic requirements of the cit2Δ mutant. The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source.",
author = "Swiegers, {Jan Hentie} and Pretorius, {Isak S.} and Bauer, {Florian F.}",
year = "2006",
month = "9",
doi = "10.1007/s00294-006-0084-z",
language = "English",
volume = "50",
pages = "161--171",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer, Springer Nature",
number = "3",

}

Regulation of respiratory growth by Ras : The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2. / Swiegers, Jan Hentie; Pretorius, Isak S.; Bauer, Florian F.

In: Current Genetics, Vol. 50, No. 3, 09.2006, p. 161-171.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Regulation of respiratory growth by Ras

T2 - Current Genetics

AU - Swiegers, Jan Hentie

AU - Pretorius, Isak S.

AU - Bauer, Florian F.

PY - 2006/9

Y1 - 2006/9

N2 - In Saccharomyces cerevisiae the Ras/cAMP/PKA signalling pathway controls multiple metabolic pathways, and alterations in the intracellular concentrations of cAMP through modification of signalling pathway factors can be lethal or result in severe growth defects. In this work, the important role of Ras2p in metabolic regulation during growth on the non-fermentable carbon source glycerol is further investigated. The data show that the overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Δ. The overexpression results in enhanced proliferation and biomass yield when cells are grown on glycerol as sole carbon source, and increases citrate synthase activity and intracellular citrate concentration. Interestingly, the suppression of cit2Δ and the enhanced proliferation and biomass yield are only observed when RAS2 is overexpressed and not in strains containing the constitutively active allele RAS2 val19. However, both RAS2 and RAS2 val19upregulated citrate synthase activity. We propose that the RAS2 overexpression results in a combination of general upregulation of respiratory growth capacity and an increase in mitochondrial citrate/ citrate synthases, which together, complement the metabolic requirements of the cit2Δ mutant. The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source.

AB - In Saccharomyces cerevisiae the Ras/cAMP/PKA signalling pathway controls multiple metabolic pathways, and alterations in the intracellular concentrations of cAMP through modification of signalling pathway factors can be lethal or result in severe growth defects. In this work, the important role of Ras2p in metabolic regulation during growth on the non-fermentable carbon source glycerol is further investigated. The data show that the overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Δ. The overexpression results in enhanced proliferation and biomass yield when cells are grown on glycerol as sole carbon source, and increases citrate synthase activity and intracellular citrate concentration. Interestingly, the suppression of cit2Δ and the enhanced proliferation and biomass yield are only observed when RAS2 is overexpressed and not in strains containing the constitutively active allele RAS2 val19. However, both RAS2 and RAS2 val19upregulated citrate synthase activity. We propose that the RAS2 overexpression results in a combination of general upregulation of respiratory growth capacity and an increase in mitochondrial citrate/ citrate synthases, which together, complement the metabolic requirements of the cit2Δ mutant. The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source.

UR - http://www.scopus.com/inward/record.url?scp=33747151594&partnerID=8YFLogxK

U2 - 10.1007/s00294-006-0084-z

DO - 10.1007/s00294-006-0084-z

M3 - Article

VL - 50

SP - 161

EP - 171

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 3

ER -