Regulation of the multiple molecular forms of rat liver glucose 6-phospnate dehydrogenase by insulin and dietary restriction

Ralph N. Martins*, Gilbert B. Stokes, Colin L. Masters

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Insulin treatment of virgin female rats increased the hepatic activity of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase to levels 3.4 and 1.5 fold higher than controls. The increase in glucose 6-phosphate dehydrogenase activity was attributed to increased activity of all three dimer species. Thus dimer bands, 1, 2 and 3 of insulin-treated animals were 5, 3 and 2-fold higher respectively than controls. The activity of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase decreased with fasting to 55% and 72% respectively of controls. The decrease in glucose 6-phosphate dehydrogenase activity reflected a lower activity of dimer bands 2 and 3 only, which were 62% and 39% of control activity respectively after three days fasting. A shift towards band 1 was observed under both conditions of starvation as well as under conditions of insulin treatment.

Original languageEnglish
Pages (from-to)136-142
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume127
Issue number1
DOIs
Publication statusPublished - 28 Feb 1985
Externally publishedYes

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