Relative quantitation of multi-antennary N-glycan classes: Combining PGC-LC-ESI-MS with exoglycosidase digestion

J. L. Abrahams; N. H. Packer; M. P. Campbell

doi:10.1039/c5an00691k

Relative quantitation of multi-antennary N-glycan classes: Combining PGC-LC-ESI-MS with exoglycosidase digestion

J. L. Abrahams, N. H. Packer^*, M. P. Campbell

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.

Original language	English
Pages (from-to)	5444-5449
Number of pages	6
Journal	Analyst
Volume	140
Issue number	16
DOIs	https://doi.org/10.1039/c5an00691k
Publication status	Published - 21 Aug 2015

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10.1039/c5an00691k

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Relative quantitation of multi-antennary N-glycan classes: Combining PGC-LC-ESI-MS with exoglycosidase digestion

Abstract

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Sugars in the real world: Are cultured cancer cells a good model system for studying protein glycosylation?

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Relative quantitation of multi-antennary N-glycan classes: Combining PGC-LC-ESI-MS with exoglycosidase digestion

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Sugars in the real world: Are cultured cancer cells a good model system for studying protein glycosylation?

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