Relative quantitation of multi-antennary N-glycan classes

Combining PGC-LC-ESI-MS with exoglycosidase digestion

J. L. Abrahams, N. H. Packer*, M. P. Campbell

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.

Original languageEnglish
Pages (from-to)5444-5449
Number of pages6
JournalAnalyst
Volume140
Issue number16
DOIs
Publication statusPublished - 21 Aug 2015

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