TY - JOUR
T1 - Repetitive element PCR fingerprinting (rep-PCR) using enterobacterial repetitive intergenic consensus (ERIC) primers is not necessarily directed at ERIC elements
AU - Gillings, M.
AU - Holley, M.
PY - 1997
Y1 - 1997
N2 - We examined the use of enterobacterial repetitive intergenic consensus (ERIC) sequences in PCR on the DNAs of various bacteria, bacteriophage, invertebrates, fungi, plants and vertebrates and have shown that complex ERIC-PCR patterns can be readily produced from all of these target organisms. A range of annealing temperatures was tested, from 52°C (the commonly used annealing temperature) to 66°C (the approximate T(m) of ERIC primers). At the higher temperatures, most bands failed to amplify, the exception being a subset of bands from enterobacterial targets. It was concluded that ERIC-PCR does not necessarily direct amplification from genuine ERIC sequences.
AB - We examined the use of enterobacterial repetitive intergenic consensus (ERIC) sequences in PCR on the DNAs of various bacteria, bacteriophage, invertebrates, fungi, plants and vertebrates and have shown that complex ERIC-PCR patterns can be readily produced from all of these target organisms. A range of annealing temperatures was tested, from 52°C (the commonly used annealing temperature) to 66°C (the approximate T(m) of ERIC primers). At the higher temperatures, most bands failed to amplify, the exception being a subset of bands from enterobacterial targets. It was concluded that ERIC-PCR does not necessarily direct amplification from genuine ERIC sequences.
UR - http://www.scopus.com/inward/record.url?scp=0030745504&partnerID=8YFLogxK
U2 - 10.1046/j.1472-765X.1997.00162.x
DO - 10.1046/j.1472-765X.1997.00162.x
M3 - Article
C2 - 9248074
AN - SCOPUS:0030745504
SN - 0266-8254
VL - 25
SP - 17
EP - 21
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 1
ER -