Resolving low-expression cell surface antigens by time-gated orthogonal scanning automated microscopy

Jie Lu, Jody Martin, Yiqing Lu, Jiangbo Zhao, Jingli Yuan, Martin Ostrowski, Ian Paulsen, James A. Piper, Dayong Jin*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report a highly sensitive method for rapid identification and quantification of rare-event cells carrying low-abundance surface biomarkers. The method applies lanthanide bioprobes and time-gated detection to effectively eliminate both nontarget organisms and background noise and utilizes the europium containing nanoparticles to further amplify the signal strength by a factor of ∼20. Of interest is that these nanoparticles did not correspondingly enhance the intensity of nonspecific binding. Thus, the dramatically improved signal-to-background ratio enables the low-expression surface antigens on single cells to be quantified. Furthermore, we applied an orthogonal scanning automated microscopy (OSAM) technique to rapidly process a large population of target-only cells on microscopy slides, leading to quantitative statistical data with high certainty. Thus, the techniques together resolved nearly all false-negative events from the interfering crowd including many false-positive events.

Original languageEnglish
Pages (from-to)9674-9678
Number of pages5
JournalAnalytical Chemistry
Volume84
Issue number22
DOIs
Publication statusPublished - 20 Nov 2012

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