TY - JOUR
T1 - Responsive upconversion nanoprobe for background-free hypochlorous acid detection and bioimaging
AU - Zhang, Run
AU - Liang, Liuen
AU - Meng, Qingtao
AU - Zhao, Jiangbo
AU - Ta, Hang T.
AU - Li, Li
AU - Zhang, Zhiqiang
AU - Sultanbawa, Yasmina
AU - Xu, Zhi Ping
PY - 2019/1/11
Y1 - 2019/1/11
N2 - Responsive nanoprobes play an important role in bioassay and bioimaging, early diagnosis of diseases and treatment monitoring. Herein, a upconversional nanoparticle (UCNP)-based nanoprobe, Ru@UCNPs, for specific sensing and imaging of hypochlorous acid (HOCl) is reported. This Ru@UCNP nanoprobe consists of two functional components,, i.e., NaYF4:Yb, Tm UCNPs that can convert near infrared light-to-visible light as the energy donor, and a HOCl-responsive ruthenium(II) complex [Ru(bpy)2(DNCH-bpy)](PF6)2 (Ru-DNPH) as the energy acceptor and also the upconversion luminescence (UCL) quencher. Within this luminescence resonance energy transfer nanoprobe system, the UCL OFF–ON emission is triggered specifically by HOCl. This triggering reaction enables the detection of HOCl in aqueous solution and biological systems. As an example of applications, the Ru@UCNPs nanoprobe is loaded onto test papers for semiquantitative HOCl detection without any interference from the background fluorescence. The application of Ru@UCNPs for background-free detection and visualization of HOCl in cells and mice is successfully demonstrated. This research has thus shown that Ru@UCNPs is a selective HOCl-responsive nanoprobe, providing a new way to detect HOCl and a new strategy to develop novel nanoprobes for in situ detection of various biomarkers in cells and early disgnosis of animal diseases.
AB - Responsive nanoprobes play an important role in bioassay and bioimaging, early diagnosis of diseases and treatment monitoring. Herein, a upconversional nanoparticle (UCNP)-based nanoprobe, Ru@UCNPs, for specific sensing and imaging of hypochlorous acid (HOCl) is reported. This Ru@UCNP nanoprobe consists of two functional components,, i.e., NaYF4:Yb, Tm UCNPs that can convert near infrared light-to-visible light as the energy donor, and a HOCl-responsive ruthenium(II) complex [Ru(bpy)2(DNCH-bpy)](PF6)2 (Ru-DNPH) as the energy acceptor and also the upconversion luminescence (UCL) quencher. Within this luminescence resonance energy transfer nanoprobe system, the UCL OFF–ON emission is triggered specifically by HOCl. This triggering reaction enables the detection of HOCl in aqueous solution and biological systems. As an example of applications, the Ru@UCNPs nanoprobe is loaded onto test papers for semiquantitative HOCl detection without any interference from the background fluorescence. The application of Ru@UCNPs for background-free detection and visualization of HOCl in cells and mice is successfully demonstrated. This research has thus shown that Ru@UCNPs is a selective HOCl-responsive nanoprobe, providing a new way to detect HOCl and a new strategy to develop novel nanoprobes for in situ detection of various biomarkers in cells and early disgnosis of animal diseases.
KW - bioassay and imaging
KW - luminescence resonance energy transfer
KW - nanoprobes
KW - paper-based test strips
KW - selective HOCl detection and imaging
UR - http://www.scopus.com/inward/record.url?scp=85058408391&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/DP170104643
U2 - 10.1002/smll.201803712
DO - 10.1002/smll.201803712
M3 - Article
C2 - 30548763
AN - SCOPUS:85058408391
SN - 1613-6810
VL - 15
SP - 1
EP - 11
JO - Small
JF - Small
IS - 2
M1 - 1803712
ER -