Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

Matthew Wilding*, Thomas S. Peat, Subha Kalyaanamoorthy, Janet Newman, Colin Scott, Lars S. Jermiin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)
17 Downloads (Pure)

Abstract

The development of new biocatalysts using ancestral sequence reconstruction is reported. When applied to an ω-transaminase, the ancestral proteins demonstrated novel and superior activities with eighty percent of the forty compounds tested compared to the modern day protein, and improvements in activity of up to twenty fold. These included a range of compounds pertinent as feedstocks in polyamide manufacture.

Original languageEnglish
Pages (from-to)5375-5380
Number of pages6
JournalGreen Chemistry
Volume19
Issue number22
DOIs
Publication statusPublished - 2017
Externally publishedYes

Bibliographical note

Copyright The Royal Society of Chemistry 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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