Abstract
We described a new ensemble-approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nm. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH-Cu2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.
Original language | English |
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Pages (from-to) | 2411-2418 |
Number of pages | 8 |
Journal | Chemistry - An Asian Journal |
Volume | 10 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1 Nov 2015 |
Keywords
- chemosensor
- fluorescence imaging
- histidine
- lysosome
- quantification