Reversible and selective fluorescence detection of histidine using a naphthalimide-based chemosensing ensemble

Qingtao Meng*, Hongmin Jia, Xue Gao, Yue Wang, Run Zhang, Renjie Wang, Zhiqiang Zhang

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We described a new ensemble-approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nm. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH-Cu2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.

Original languageEnglish
Pages (from-to)2411-2418
Number of pages8
JournalChemistry - An Asian Journal
Volume10
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015

Keywords

  • chemosensor
  • fluorescence imaging
  • histidine
  • lysosome
  • quantification

Fingerprint Dive into the research topics of 'Reversible and selective fluorescence detection of histidine using a naphthalimide-based chemosensing ensemble'. Together they form a unique fingerprint.

  • Cite this