Reversible and selective fluorescence detection of histidine using a naphthalimide-based chemosensing ensemble

Qingtao Meng*, Hongmin Jia, Xue Gao, Yue Wang, Run Zhang, Renjie Wang, Zhiqiang Zhang

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    We described a new ensemble-approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nm. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH-Cu2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.

    Original languageEnglish
    Pages (from-to)2411-2418
    Number of pages8
    JournalChemistry - An Asian Journal
    Volume10
    Issue number11
    DOIs
    Publication statusPublished - 1 Nov 2015

    Keywords

    • chemosensor
    • fluorescence imaging
    • histidine
    • lysosome
    • quantification

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