Reversible energy-transfer switching on a DNA scaffold

Magnus Bälter; Martin Hammarson; Patricia Remón; Shiming Li; Nittaya Gale; Tom Brown; Joakim Andréasson

doi:10.1021/ja512416n

Reversible energy-transfer switching on a DNA scaffold

Magnus Bälter, Martin Hammarson, Patricia Remón, Shiming Li, Nittaya Gale, Tom Brown, Joakim Andréasson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

18 Downloads (Pure)

Abstract

We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

Original language	English
Pages (from-to)	2444-2447
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	137
Issue number	7
DOIs	https://doi.org/10.1021/ja512416n
Publication status	Published - 25 Feb 2015
Externally published	Yes

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Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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abstract = "We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.",

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AU - Bälter, Magnus

AU - Hammarson, Martin

AU - Remón, Patricia

AU - Li, Shiming

AU - Gale, Nittaya

AU - Brown, Tom

AU - Andréasson, Joakim

N1 - Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2015/2/25

Y1 - 2015/2/25

N2 - We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

AB - We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

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Reversible energy-transfer switching on a DNA scaffold

Abstract

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