Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

E. V. Khaydukov, K. E. Mironova, V. A. Semchishen, A. N. Generalova, A. V. Nechaev, D. A. Khochenkov, E. V. Stepanova, O. I. Lebedev, A. V. Zvyagin*, S. M. Deyev, V. Ya Panchenko

*Corresponding author for this work

Research output: Contribution to journalArticle

42 Citations (Scopus)
14 Downloads (Pure)

Abstract

Riboflavin (Rf) is a vitamin and endogenous photosensitizer capable to generate reactive oxygen species (ROS) under UV-blue irradiation and kill cancer cells, which are characterized by the enhanced uptake of Rf. We confirmed its phototoxicity on human breast adenocarcinoma cells SK-BR-3 preincubated with 30-μM Rf and irradiated with ultraviolet light, and proved that such Rf concentrations (60 μM) are attainable in vivo in tumour site by systemic intravascular injection. In order to extend the Rf photosensitization depth in cancer tissue to 6 mm in depth, we purpose-designed core/shell upconversion nanoparticles (UCNPs, NaYF4:Yb3+:Tm3+/NaYF4) capable to convert 2% of the deeply-penetrating excitation at 975 nm to ultraviolet-blue power. This power was expended to photosensitise Rf and kill SK-BR-3 cells preincubated with UCNPs and Rf, where the UCNP-Rf energy transfer was photon-mediated with ∼14% Förster process contribution. SK-BR-3 xenograft regression in mice was observed for 50 days, following the Rf-UCNPs peritumoural injection and near-infrared light photodynamic treatment of the lesions.

Original languageEnglish
Article number35103
Pages (from-to)1-9
Number of pages9
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 12 Oct 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Fingerprint Dive into the research topics of 'Riboflavin photoactivation by upconversion nanoparticles for cancer treatment'. Together they form a unique fingerprint.

  • Cite this

    Khaydukov, E. V., Mironova, K. E., Semchishen, V. A., Generalova, A. N., Nechaev, A. V., Khochenkov, D. A., ... Panchenko, V. Y. (2016). Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. Scientific Reports, 6, 1-9. [35103]. https://doi.org/10.1038/srep35103