Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6

Research output: Research - peer-reviewArticle

Abstract

Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL−1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4 pg mL−1 to 400 pg mL−1 of IL-6, with the limit of detection down to 0.1 pg mL−1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200 μm and a sample volume of 1 μL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).
LanguageEnglish
Pages80-86
Number of pages7
JournalBiosensors and Bioelectronics
Volume102
DOIs
StatePublished - 2018

Fingerprint

Streptavidin
Biotin
Interleukin-6
Antibodies
Fibers
Immunosensors
Fiber optic sensors
Optical Fibers
Cell culture
Optical fibers
Assays
Enzymes
Fluorescence
Nanoparticles
Fluids
Sensors
Immobilized Antibodies
Immunosorbents
Culture Media
Limit of Detection

Cite this

@article{a9927f1b2c724d0994dc6df817736bae,
title = "Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6",
abstract = "Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL−1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4 pg mL−1 to 400 pg mL−1 of IL-6, with the limit of detection down to 0.1 pg mL−1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200 μm and a sample volume of 1 μL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).",
keywords = "optical fibre, cytokine assay, biotin-streptavidin, interleukin-6, immunosensor",
author = "Kaixin Zhang and Guozhen Liu and Ewa Goldys",
year = "2018",
doi = "10.1016/j.bios.2017.11.023",
volume = "102",
pages = "80--86",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Robust immunosensing system based on biotin-streptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6

AU - Zhang,Kaixin

AU - Liu,Guozhen

AU - Goldys,Ewa

PY - 2018

Y1 - 2018

N2 - Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL−1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4 pg mL−1 to 400 pg mL−1 of IL-6, with the limit of detection down to 0.1 pg mL−1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200 μm and a sample volume of 1 μL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).

AB - Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL−1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4 pg mL−1 to 400 pg mL−1 of IL-6, with the limit of detection down to 0.1 pg mL−1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200 μm and a sample volume of 1 μL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).

KW - optical fibre

KW - cytokine assay

KW - biotin-streptavidin

KW - interleukin-6

KW - immunosensor

U2 - 10.1016/j.bios.2017.11.023

DO - 10.1016/j.bios.2017.11.023

M3 - Article

VL - 102

SP - 80

EP - 86

JO - Biosensors and Bioelectronics

T2 - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -