Role of dnaB43 in F′-plasmid incompatibility

Alan F. Jamieson*, Peter L. Bergquist

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In order to perform complementation tests with mutations of DNA replication of F′-plasmids incompatibility must be overcome. We report our inability to duplicate the results presented by Palchoudhury and Iyer (1971) and Bezanson and Iyer (1975) who have claimed to demonstrate the autonomous replication of two incompatible F′-plasmids in a strain carrying the temperature sensitive dnaB43 allele. In addition, we describe experiments designed to measure complementation using transient heterozygote and compatible plasmids. Assessment of our data and those of others in the light of a recent report by Uhlin and Nordström (1975) suggests that new approaches will have to be developed for the successful employment of complementation analysis in F-plasmid genetics.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalMGG Molecular & General Genetics
Volume150
Issue number2
DOIs
Publication statusPublished - Jan 1977
Externally publishedYes

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