Role of the NLRP3 inflammasome in a model of acute burn-induced pain

Jennifer R. Deuis, Kathleen Yin, Matthew A. Cooper, Kate Schroder, Irina Vetter*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The NLRP3 inflammasome is a multi-protein complex that assembles in response to tissue damage or infection, triggering activation of caspase-1, an enzyme that converts interleukin (IL)-1β into its active form. A role for the NLRP3 inflammasome is emerging in inflammatory pain, but its influence in other pain types is largely unexamined. Therefore the aim of this study was to assess the role of the NLRP3 inflammasome and its downstream product caspase-1 in a model of acute burn-induced pain in male mice. A superficial burn was induced on the plantar surface of the left hind paw using a hot plate set at 52.5 °C for 25 s. Development of burn-induced mechanical allodynia, thermal allodynia, edema and weight bearing changes was assessed in Nlrp3−/− and caspase-1-deficient (Ice−/−) mice, and in mice administered the selective NLRP3 inflammasome inhibitor MCC950. Burn-induced mechanical and thermal allodynia developed normally in Nlrp3−/− and Ice−/− mice and mice administered MCC950. Burn-induced edema was significantly reduced in Ice−/− mice only. Burn-induced weight bearing changes were attenuated in Nlrp3−/− mice and mice administered MCC950 72 h after burn only. This study suggests that NLRP3 and its downstream product caspase-1 have a limited role in the development of burn-induced pain.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalBurns
Volume43
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Keywords

  • NLRP3 inflammasome
  • caspase-1
  • interleukin-1β
  • burns
  • mouse model
  • pain

Fingerprint Dive into the research topics of 'Role of the NLRP3 inflammasome in a model of acute burn-induced pain'. Together they form a unique fingerprint.

  • Cite this

    Deuis, J. R., Yin, K., Cooper, M. A., Schroder, K., & Vetter, I. (2017). Role of the NLRP3 inflammasome in a model of acute burn-induced pain. Burns, 43(2), 304-309. https://doi.org/10.1016/j.burns.2016.09.001