Rostroventrolateral medulla neurons with commissural projections provide input to sympathetic premotor neurons

anatomical and functional evidence

Anita Turner, Natasha Kumar, Melissa Farnham, Mandy Lung, Paul Pilowsky, Simon Mcmullan*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The activity of neurons in the rostral ventrolateral medulla (RVLM) is critical for the generation of vasomotor sympathetic tone. Multiple pre-sympathetic pathways converge on spinally projecting RVLM neurons, but the origin and circumstances in which such inputs are active are poorly understood. We have previously shown that input from the contralateral brainstem contributes to the baseline activity of this population: in the current study we investigate the distribution, phenotype and functional properties of RVLM neurons with commissural projections in the rat. We firstly used retrograde transport of fluorescent microspheres to identify neurons that project to the contralateral RVLM. Labelled neurons were prominent in a longitudinal column that extended over 1 mm caudal from the facial nucleus and contained hybridisation products indicating enkephalin (27%), GABA (15%) and adrenaline (3%) synthesis and included 6% of bulbospinal neurons identified by transport of cholera toxin B. Anterograde transport of fluorescent dextran-conjugate from the contralateral RVLM revealed extensive inputs throughout the RVLM that frequently terminated in close apposition with catecholaminergic and bulbospinal neurons. In urethane-anaesthetised rats we verified that 28/37 neurons antidromically activated by electrical stimulation of the contralateral pressor region were spontaneously active, of which 13 had activity locked to central respiratory drive and 15 displayed ongoing tonic discharge. In six tonically active neurons sympathoexcitatory roles were indicated by spike-triggered averages of splanchnic sympathetic nerve activity. We conclude that neurons in the RVLM project to the contralateral brainstem, form synapses with sympathetic premotor neurons, and have functional properties consistent with sympthoexcitatory function.

    Original languageEnglish
    Pages (from-to)2504-2515
    Number of pages12
    JournalEuropean Journal of Neuroscience
    Volume38
    Issue number4
    DOIs
    Publication statusPublished - Aug 2013

    Keywords

    • anterograde
    • extracellular single unit
    • retrograde
    • spike-triggered average
    • sympathetic neuron

    Fingerprint Dive into the research topics of 'Rostroventrolateral medulla neurons with commissural projections provide input to sympathetic premotor neurons: anatomical and functional evidence'. Together they form a unique fingerprint.

  • Cite this