S100A8 induces IL-10 and protects against acute lung injury

Yuka Hiroshima, Kenneth Hsu, Nicodemus Tedla, Yuen Ming Chung, Sharron Chow, Cristan Herbert, Carolyn L. Geczy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

S100A8 is considered proinflammatory by activating TLR4 and/or the receptor for advanced glycation end products. The aim was to investigate inflammatory effects of S100A8 in murine lung. S100A8 was administered to BALB/c mice by nasal inhalation and genes induced over a time-course assessed. LPS was introduced intranasally either alone or 2 h after pretreatment of mice with intranasal application of S100A8 or dexamethasone. A Cys(42)-Ala(42) mutant S100A8 mutant was used to assess whether S100A8's effects were via pathways that were dependent on reactive oxygen species. S100A8 induced IL-10 mRNA, and expression was apparent only in airway epithelial cells. Importantly, it suppressed acute lung injury provoked by LPS inhalation by suppressing mast-cell activation and induction of mediators orchestrating leukocyte recruitment, possibly by reducing NF-kappa B activation via an I kappa B alpha/Akt pathway and by downmodulating pathways generating oxidative stress. The Cys(42)-Ala(42) S100A8 mutant did not induce IL-10 and was less immunosuppressive, indicating modulation by scavenging oxidants. S100A8 inhibition of LPS-mediated injury was as potent, and outcomes were remarkably similar to immunosuppression by dexamethasone. We challenge the notion that S100A8 is an agonist for TLR4 or the receptor for advanced glycation end products. S100A8 induced IL-10 in vivo and initiates a feedback loop that attenuates acute lung injury.

Original languageEnglish
Pages (from-to)2800-2811
Number of pages12
JournalJournal of Immunology
Volume192
Issue number6
DOIs
Publication statusPublished - 15 Mar 2014
Externally publishedYes

Keywords

  • KAPPA-B ACTIVATION
  • ANTIINFLAMMATORY PROPERTIES
  • CHEMOTACTIC PROTEIN
  • N-ACETYLCYSTEINE
  • IN-VIVO
  • INFLAMMATION
  • ENDOTOXIN
  • INHIBITION
  • CELLS
  • CP-10

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