Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product

David J. Thornton*, Nagma Khan, Ravi Mehrotra, Marj Howard, Enno Veerman, Nicolle H. Packer, John K. Sheehan

*Corresponding author for this work

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm) and molecular weight (2.5-2.9 x 106 Da). Two differently-charged populations of MG1 subunits were observed which showed different reactivity with monoclonal antibodies to glycan epitopes. Monosaccharide and amino acid compositional analyses indicated that the MG1 subunits had similar glycan structures on the same polypeptide. An antiserum recognizing the MUC5B mucin was reactive across the entire distribution, whereas antisera raised against the MUC2 and MUC5AC mucins showed no reactivity. Western blots of agarose gel electrophoresis of fractions across the anion exchange distribution indicated that the polypeptide underlying the mucins was the product of the MUC5B gene. Amino acid analysis and peptide mapping performed on the fragments produced by trypsin digestion of the two MG1 populations yielded data similar to that obtained for MUC5B mucin subunits prepared from respiratory mucus and confirmed that the MUC5B gene product was the predominant mucin polypeptide present. Isolation of the MG1 mucins from the secretions of the individual salivary glands (palatal, sublingual, and submandibular) indicate that the palatal gland is the source of the highly charged population of the MUC5B mucin.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalGlycobiology
Volume9
Issue number3
Publication statusPublished - Mar 1999

Keywords

  • MUC5B
  • Mucin
  • Saliva
  • Salivary glands

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    Thornton, D. J., Khan, N., Mehrotra, R., Howard, M., Veerman, E., Packer, N. H., & Sheehan, J. K. (1999). Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Glycobiology, 9(3), 293-302.