Salt-treated roots of Oryza australiensis seedlings are enriched with proteins involved in energetics and transport

Yoav Yichie, Mafruha T. Hasan, Peri A. Tobias, Dana Pascovici, Hugh D. Goold, Steven C. Van Sluyter, Thomas H. Roberts, Brian J. Atwell

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Salinity is a major constraint on rice productivity worldwide. However, mechanisms of salt tolerance in wild rice relatives are unknown. Root microsomal proteins are extracted from two Oryza australiensis accessions contrasting in salt tolerance. Whole roots of 2-week-old seedlings are treated with 80 mM NaCl for 30 days to induce salt stress. Proteins are quantified by tandem mass tags (TMT) and triple-stage Mass Spectrometry. More than 200 differentially expressed proteins between the salt-treated and control samples in the two accessions (p-value <0.05) are found. Gene Ontology (GO) analysis shows that proteins categorized as "metabolic process," "transport," and "transmembrane transporter" are highly responsive to salt treatment. In particular, mitochondrial ATPases and SNARE proteins are more abundant in roots of the salt-tolerant accession and responded strongly when roots are exposed to salinity. mRNA quantification validated the elevated protein abundances of a monosaccharide transporter and an antiporter observed in the salt-tolerant genotype. The importance of the upregulated monosaccharide transporter and a VAMP-like protein by measuring salinity responses of two yeast knockout mutants for genes homologous to those encoding these proteins in rice are confirmed. Potential new mechanisms of salt tolerance in rice, with implications for breeding of elite cultivars are also discussed.

    Original languageEnglish
    Article number1900175
    Number of pages12
    JournalProteomics
    Volume19
    Issue number19
    DOIs
    Publication statusPublished - Oct 2019

    Keywords

    • membrane proteins
    • Oryza australiensis
    • plant proteomics
    • rice
    • salt tolerance

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