Schlieren confocal microscopy for phase-relief imaging

Hao Xie; Dayong Jin; Junjie Yu; Tong Peng; Yichen Ding; Changhe Zhou; Peng Xi

doi:10.1364/OL.39.001238

Schlieren confocal microscopy for phase-relief imaging

Hao Xie, Dayong Jin, Junjie Yu, Tong Peng, Yichen Ding, Changhe Zhou, Peng Xi^*

^*Corresponding author for this work

MQ Photonics Research Centre

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system.

Original language	English
Pages (from-to)	1238-1241
Number of pages	4
Journal	Optics Letters
Volume	39
Issue number	5
DOIs	https://doi.org/10.1364/OL.39.001238
Publication status	Published - 1 Mar 2014

Bibliographical note

This paper was published in Optics Letters and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-39-5-1238. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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title = "Schlieren confocal microscopy for phase-relief imaging",

abstract = "We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system.",

author = "Hao Xie and Dayong Jin and Junjie Yu and Tong Peng and Yichen Ding and Changhe Zhou and Peng Xi",

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AU - Ding, Yichen

AU - Zhou, Changhe

AU - Xi, Peng

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Schlieren confocal microscopy for phase-relief imaging

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