TY - JOUR
T1 - Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
AU - International Stem Cell Initiative
AU - Amps, Katherine
AU - Andrews, Peter W.
AU - Anyfantis, George
AU - Armstrong, Lyle
AU - Avery, Stuart
AU - Baharvand, Hossein
AU - Baker, Julie
AU - Baker, Duncan
AU - Munoz, Maria B.
AU - Beil, Stephen
AU - Benvenisty, Nissim
AU - Ben-Yosef, Dalit
AU - Biancotti, Juan Carlos
AU - Bosman, Alexis
AU - Brena, Romulo Martin
AU - Brison, Daniel
AU - Caisander, Gunilla
AU - Camarasa, Marãa V.
AU - Chen, Jieming
AU - Chiao, Eric
AU - Choi, Young Min
AU - Choo, Andre B. H.
AU - Collins, Daniel
AU - Colman, Alan
AU - Crook, Jeremy M.
AU - Daley, George Q.
AU - Dalton, Anne
AU - De Sousa, Paul A.
AU - Denning, Chris
AU - Downie, Janet
AU - Dvorak, Petr
AU - Montgomery, Karen D.
AU - Feki, Anis
AU - Ford, Angela
AU - Fox, Victoria
AU - Fraga, Ana M.
AU - Frumkin, Tzvia
AU - Ge, Lin
AU - Gokhale, Paul J.
AU - Golan-Lev, Tamar
AU - Gourabi, Hamid
AU - Gropp, Michal
AU - Guangxiu, Lu
AU - Hampl, Ales
AU - Harron, Katie
AU - Healy, Lyn
AU - Herath, Wishva
AU - Holm, Frida
AU - Hovatta, Outi
AU - Hyllner, Johan
AU - Inamdar, Maneesha S.
AU - Irwanto, Astrid Kresentia
AU - Ishii, Tetsuya
AU - Jaconi, Marisa
AU - Jin, Ying
AU - Kimber, Susan
AU - Kiselev, Sergey
AU - Knowles, Barbara B.
AU - Kopper, Oded
AU - Kukharenko, Valeri
AU - Kuliev, Anver
AU - Lagarkova, Maria A.
AU - Laird, Peter W.
AU - Lako, Majlinda
AU - Laslett, Andrew L.
AU - Lavon, Neta
AU - Lee, Dong Ryul
AU - Lee, Jeoung Eun
AU - Li, Chunliang
AU - Lim, Linda S.
AU - Ludwig, Tenneille E.
AU - Ma, Yu
AU - Maltby, Edna
AU - Mateizel, Ileana
AU - Mayshar, Yoav
AU - Mileikovsky, Maria
AU - Minger, Stephen L.
AU - Miyazaki, Takamichi
AU - Moon, Shin Yong
AU - Moore, Harry
AU - Mummery, Christine
AU - Nagy, Andras
AU - Nakatsuji, Norio
AU - Narwani, Kavita
AU - Oh, Steve K. W.
AU - Oh, Sun Kyung
AU - Olson, Cia
AU - Otonkoski, Timo
AU - Pan, Fei
AU - Park, In Hyun
AU - Pells, Steve
AU - Pera, Martin F.
AU - Pereira, Lygia V.
AU - Qi, Ouyang
AU - Raj, Grace Selva
AU - Reubinoff, Benjamin
AU - Robins, Alan
AU - Robson, Paul
AU - Rossant, Janet
AU - Salekdeh, Ghasem H.
AU - Schulz, Thomas C.
AU - Sermon, Karen
AU - Mohamed, Jameelah Sheik
AU - Shen, Hui
AU - Sherrer, Eric
AU - Sidhu, Kuldip
AU - Sivarajah, Shirani
AU - Skottman, Heli
AU - Spits, Claudia
AU - Stacey, Glyn N.
AU - Strehl, Raimund
AU - Strelchenko, Nick
AU - Suemori, Hirofumi
AU - Sun, Bowen
AU - Suuronen, Riitta
AU - Takahashi, Kazutoshi
AU - Tuuri, Timo
AU - Venu, Parvathy
AU - Verlinsky, Yuri
AU - Oostwaard, Dorien Ward Van
AU - Weisenberger, Daniel J.
AU - Wu, Yue
AU - Yamanaka, Shinya
AU - Young, Lorraine
AU - Zhou, Qi
PY - 2011/12/1
Y1 - 2011/12/1
N2 - The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
AB - The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
UR - http://www.scopus.com/inward/record.url?scp=83255189758&partnerID=8YFLogxK
U2 - 10.1038/nbt.2051
DO - 10.1038/nbt.2051
M3 - Article
C2 - 22119741
AN - SCOPUS:83255189758
SN - 1087-0156
VL - 29
SP - 1132
EP - 1144
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 12
ER -