Selection of DNA aptamers that bind to four organophosphorus pesticides

Li Wang, Xianjin Liu, Qiang Zhang, Cunzheng Zhang, Yuan Liu, Kang Tu*, Jian Tu

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    84 Citations (Scopus)

    Abstract

    Single-stranded DNA (ssDNA) aptamers against four organophosphorus pesticides (phorate, profenofos, isocarbophos and omethoate) were simultaneously isolated from an immobilized random ssDNA library by systematic evolution of ligands by exponential enrichment (SELEX) technique. After 12 rounds of in vitro selection, five ssDNA aptamer candidates were selected and their binding affinities were identified by a novel method using a molecular beacon. Two of the five ssDNA sequences, SS2-55 and SS4-54, demonstrated higher affinities and specificities to the four organophosphorus pesticides. They were defined as broad-spectrum aptamers binding to four different targets and their simulated secondary structures showed highly distinct features with typical stem and loop structures. The dissociation constant of SS2-55 and SS4-54 binding to the four organophosphorus pesticides ranged from 0. 8 to 2. 5 μM. These aptamers offered application potential in the analysis and/or neutralization of the residues of the four organophosphorus pesticides.

    Original languageEnglish
    Pages (from-to)869-874
    Number of pages6
    JournalBiotechnology Letters
    Volume34
    Issue number5
    DOIs
    Publication statusPublished - May 2012

    Keywords

    • DNA aptamer
    • Food safety monitoring and analysis
    • Molecular beacon
    • Organophosphorus pesticide
    • SELEX

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