TY - JOUR
T1 - Selective recognition of cis-trans-isomers of platinum drugs and the detection of triplex DNA based on fluorescence reversible model of quantum dots
AU - Xu, Xiaoling
AU - Gao, Fang
AU - Xiao, Xincai
AU - Hu, Yan
AU - Zhu, Chaozhen
AU - Zhao, Dan
PY - 2017/2/5
Y1 - 2017/2/5
N2 - The identification of spatial structures of drugs and the researches on their interaction mechanism with DNA are always attractive to the researchers. However, their realization is lack of simple and fast method. This paper reports the establishment of multiple-functional detection platform based on the “turn off-on” model of ZnCdSe quantum dots. In this system, ZnCdSe quantum dots work as the fluorescent probe, platinum anti-cancer drugs as the quencher and triplex DNA as the trapping agent. The seemingly similar cisplatin and transplatin exhibited different fluorescent recovery behaviors due to their difference in structure, and thus realized the selective detection of cisplatin and transplatin with the reaction time set at 10 min as well as the quantitation of cisplatin over the range of 2.5 × 10−8–100 × 10−8M. Based on this, the interactions between platinum anti-cancer drugs and ctDNA as well as polymorphic DNA were further studied, and realized the recognition of triplex DNA. The multiple-functional detection platform integrates the functions of the filtration of high-efficient platinum anti-cancer drugs, the researches on interaction mechanism of drugs, and the recognition of polymorphic DNA, meaningful to the future treatment of viral and cancers based on antisense gene strategy.
AB - The identification of spatial structures of drugs and the researches on their interaction mechanism with DNA are always attractive to the researchers. However, their realization is lack of simple and fast method. This paper reports the establishment of multiple-functional detection platform based on the “turn off-on” model of ZnCdSe quantum dots. In this system, ZnCdSe quantum dots work as the fluorescent probe, platinum anti-cancer drugs as the quencher and triplex DNA as the trapping agent. The seemingly similar cisplatin and transplatin exhibited different fluorescent recovery behaviors due to their difference in structure, and thus realized the selective detection of cisplatin and transplatin with the reaction time set at 10 min as well as the quantitation of cisplatin over the range of 2.5 × 10−8–100 × 10−8M. Based on this, the interactions between platinum anti-cancer drugs and ctDNA as well as polymorphic DNA were further studied, and realized the recognition of triplex DNA. The multiple-functional detection platform integrates the functions of the filtration of high-efficient platinum anti-cancer drugs, the researches on interaction mechanism of drugs, and the recognition of polymorphic DNA, meaningful to the future treatment of viral and cancers based on antisense gene strategy.
KW - Cis-trans-isomers
KW - Fluorescence
KW - Quantum dots
KW - Triplex DNA
UR - http://www.scopus.com/inward/record.url?scp=84998842327&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2016.11.033
DO - 10.1016/j.jpba.2016.11.033
M3 - Article
C2 - 27889670
AN - SCOPUS:84998842327
SN - 0731-7085
VL - 134
SP - 94
EP - 99
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -