Sensitive and multiplexed SERS nanotags for the detection of cytokines secreted by lymphoma

Dan Li, Lianmei Jiang, James A. Piper, Ivan S. Maksymov, Andrew D. Greentree, Erkang Wang, Yuling Wang

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The sensitive and simultaneous detection of cytokines will provide new insights into the physiological process and disease pathways due to the complex nature of cytokine networks. However, the key challenge is the lack of probes that can simultaneously detect multiple cytokines in a single sample. In this contribution, we proposed an alternative approach for sensitive cytokine detection in a multiplex manner by the use of a new set of surface-enhanced Raman spectroscopy (SERS) nanotags. Typically, the newly designed SERS nanotags are composed of gold nanoparticles as the core, tuneable Raman molecules as the reporters, and a thin silver layer as the shell. As demonstrated through rigorous numerical simulations, enhanced Raman signal is achieved due to a strong localization of light in the 0.2 nm thin, optically deep-subwavelength region between the Au core and the Ag shell. Sensitive detection of cytokines is realized by forming a sandwich immunoassay. The detection limit is down to 4.5 pg mL-1 (S/N = 3). The specificity of the assay is proved as negligible signals were detected for the false targets. Furthermore, multiple cytokines are simultaneously detected in a single assay from the secretion of B-lymphocyte cell line (Raji) after concanavalin A (Con A) stimulation. The results indicate that our method holds a significant potential for sensitive and multiplexed detection of cytokines and offers the opportunity for future applications in clinical settings.

LanguageEnglish
Pages2507-2514
Number of pages8
JournalACS Sensors
Volume4
Issue number9
DOIs
Publication statusPublished - 27 Sep 2019

Fingerprint

Raman spectroscopy
Cytokines
immunoassay
secretions
Assays
lymphocytes
stimulation
cultured cells
Lymphocytes
silver
Concanavalin A
Silver
gold
Gold
nanoparticles
probes
Cells
Nanoparticles
Molecules
molecules

Keywords

  • surface-enhanced Raman spectroscopy
  • nanotags
  • theoretical simulation
  • multiplexed detection
  • cytokine
  • lymphoma

Cite this

Li, Dan ; Jiang, Lianmei ; Piper, James A. ; Maksymov, Ivan S. ; Greentree, Andrew D. ; Wang, Erkang ; Wang, Yuling. / Sensitive and multiplexed SERS nanotags for the detection of cytokines secreted by lymphoma. In: ACS Sensors. 2019 ; Vol. 4, No. 9. pp. 2507-2514.
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Sensitive and multiplexed SERS nanotags for the detection of cytokines secreted by lymphoma. / Li, Dan; Jiang, Lianmei; Piper, James A.; Maksymov, Ivan S.; Greentree, Andrew D.; Wang, Erkang; Wang, Yuling.

In: ACS Sensors, Vol. 4, No. 9, 27.09.2019, p. 2507-2514.

Research output: Contribution to journalArticleResearchpeer-review

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