Sensitive electrochemical determination of α-fetoprotein using a glassy carbon electrode modified with in-situ grown gold nanoparticles, graphene oxide and MWCNTs acting as signal amplifiers

Yan-Sha Gao, Xiao-Fei Zhu, Tao-Tao Yang, Jing-Kun Xu, Li-Min Lu, Kai-Xin Zhang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The authors describe an electrochemical immunoassay for α-fetoprotein (α-FP) using a glassy carbon electrode (GCE) modified with a nanocomposite made from gold nanoparticles, graphene oxide and multi-walled carbon nanotubes (AuNPs/GO-MWCNTs) and acting as a signal amplification matrix. The nanocomposite was synthesized in a one-pot redox reaction between GO and HAuCl4 without using an additional reductant. The stepwise assembly of the immunoelectrode was characterized by means of cyclic voltammetry and electrochemical impedance spectroscopy. The interaction of antigen and antibody on the surface of the electrode creates a barrier for electrons and causes retarded electron transfer, this resulting in decreased signals in differential pulse voltammetry of hexacyanoferrate which is added as an electrochemical probe. Using this strategy and by working at a potential of 0.2 V (vs. SCE), a wide analytical range (0.01 - 100 ng∙mL‾1) is covered. The correlation coefficient is 0.9929, and the limit of detection is as low as 3 pg∙mL‾1 at a signal-to-noise ratio of 3. This electrochemical immunoassay combines the specificity of an immunological detection scheme with the sensitivity of an electrode modified with AuNPs and GO-MWCNTs.

Original languageEnglish
Pages (from-to)2027-2035
Number of pages9
JournalMicrochimica Acta
Volume182
Issue number11-12
DOIs
Publication statusPublished - 1 Aug 2015
Externally publishedYes

Keywords

  • Electrochemical immunosensor
  • Gold nanoparticles
  • Graphene oxide
  • Multi-walled carbon nanotubes
  • α-fetoprotein

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