Projects per year
We describe the application of a synthetically developed tetradentate β-diketonate-europium chelate with high quantum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labeling. For all prepared conjugates, the europium chelate in conjunction with a gated autosynchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.
|Number of pages||8|
|Publication status||Published - 4 Oct 2016|
FingerprintDive into the research topics of 'Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand'. Together they form a unique fingerprint.
- 1 Finished
Rapid detection of rare-event cells by SUPER Dots: finding a needle in a haystack
Packer, N., Jin, D., Piper, J., Willows, R., Foote, S., Walsh, B., Dubljevic, V., MQRES, M. & CSC-MQRES (International), C.
12/09/13 → 11/09/16