Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Nima Sayyadi; Irene Justiniano; Russell E. Connally; Run Zhang; Bingyang Shi; Liisa Kautto; Arun V. Everest-Dass; Jingli Yuan; Bradley J. Walsh; Dayong Jin; Robert D. Willows; James A. Piper; Nicolle H. Packer

doi:10.1021/acs.analchem.6b02191

Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Nima Sayyadi^*, Irene Justiniano, Russell E. Connally, Run Zhang, Bingyang Shi, Liisa Kautto, Arun V. Everest-Dass, Jingli Yuan, Bradley J. Walsh, Dayong Jin, Robert D. Willows, James A. Piper, Nicolle H. Packer

^*Corresponding author for this work

ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP)

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We describe the application of a synthetically developed tetradentate β-diketonate-europium chelate with high quantum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labeling. For all prepared conjugates, the europium chelate in conjunction with a gated autosynchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.

Original language	English
Pages (from-to)	9564-9571
Number of pages	8
Journal	Analytical Chemistry
Volume	88
Issue number	19
DOIs	https://doi.org/10.1021/acs.analchem.6b02191
Publication status	Published - 4 Oct 2016

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Sayyadi, N., Justiniano, I., Connally, R. E., Zhang, R., Shi, B., Kautto, L., Everest-Dass, A. V., Yuan, J., Walsh, B. J., Jin, D., Willows, R. D., Piper, J. A., & Packer, N. H. (2016). Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand. Analytical Chemistry, 88(19), 9564-9571. https://doi.org/10.1021/acs.analchem.6b02191

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title = "Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand",

abstract = "We describe the application of a synthetically developed tetradentate β-diketonate-europium chelate with high quantum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labeling. For all prepared conjugates, the europium chelate in conjunction with a gated autosynchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.",

author = "Nima Sayyadi and Irene Justiniano and Connally, {Russell E.} and Run Zhang and Bingyang Shi and Liisa Kautto and Everest-Dass, {Arun V.} and Jingli Yuan and Walsh, {Bradley J.} and Dayong Jin and Willows, {Robert D.} and Piper, {James A.} and Packer, {Nicolle H.}",

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AU - Sayyadi, Nima

AU - Justiniano, Irene

AU - Connally, Russell E.

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AU - Shi, Bingyang

AU - Kautto, Liisa

AU - Everest-Dass, Arun V.

AU - Yuan, Jingli

AU - Walsh, Bradley J.

AU - Jin, Dayong

AU - Willows, Robert D.

AU - Piper, James A.

AU - Packer, Nicolle H.

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AB - We describe the application of a synthetically developed tetradentate β-diketonate-europium chelate with high quantum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labeling. For all prepared conjugates, the europium chelate in conjunction with a gated autosynchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.

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Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Abstract

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Rapid detection of rare-event cells by SUPER Dots: finding a needle in a haystack

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Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Abstract

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Rapid detection of rare-event cells by SUPER Dots: finding a needle in a haystack

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