Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes

J. M. Gebicki*, Simon Freed

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nucleotides obtained from alkaline hydrolysis of yeast and tissue RNA were separated by a 15-min electrophoresis on cellulose acetate membranes and assayed spectrophotometrically in microcells. The lower limit for analysis of each nucleotide was about 0.15 μg and the reproducibility of measurement was ±3%.

Original languageEnglish
Pages (from-to)505-509
Number of pages5
JournalAnalytical Biochemistry
Volume13
Issue number3
Publication statusPublished - Dec 1965
Externally publishedYes

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