Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes

J. M. Gebicki; Simon Freed

Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes

J. M. Gebicki^*, Simon Freed

^*Corresponding author for this work

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Abstract

Nucleotides obtained from alkaline hydrolysis of yeast and tissue RNA were separated by a 15-min electrophoresis on cellulose acetate membranes and assayed spectrophotometrically in microcells. The lower limit for analysis of each nucleotide was about 0.15 μg and the reproducibility of measurement was ±3%.

Original language	English
Pages (from-to)	505-509
Number of pages	5
Journal	Analytical Biochemistry
Volume	13
Issue number	3
Publication status	Published - Dec 1965
Externally published	Yes

Cite this

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title = "Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes",

abstract = "Nucleotides obtained from alkaline hydrolysis of yeast and tissue RNA were separated by a 15-min electrophoresis on cellulose acetate membranes and assayed spectrophotometrically in microcells. The lower limit for analysis of each nucleotide was about 0.15 μg and the reproducibility of measurement was ±3\%.",

author = "Gebicki, \{J. M.\} and Simon Freed",

year = "1965",

month = dec,

language = "English",

volume = "13",

pages = "505--509",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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T1 - Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes

AU - Gebicki, J. M.

AU - Freed, Simon

PY - 1965/12

Y1 - 1965/12

N2 - Nucleotides obtained from alkaline hydrolysis of yeast and tissue RNA were separated by a 15-min electrophoresis on cellulose acetate membranes and assayed spectrophotometrically in microcells. The lower limit for analysis of each nucleotide was about 0.15 μg and the reproducibility of measurement was ±3%.

AB - Nucleotides obtained from alkaline hydrolysis of yeast and tissue RNA were separated by a 15-min electrophoresis on cellulose acetate membranes and assayed spectrophotometrically in microcells. The lower limit for analysis of each nucleotide was about 0.15 μg and the reproducibility of measurement was ±3%.

UR - https://www.scopus.com/pages/publications/0013840140

M3 - Article

C2 - 5881306

AN - SCOPUS:0013840140

SN - 0003-2697

VL - 13

SP - 505

EP - 509

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 3

ER -

Separation of nucleotides from microgram amounts of RNA by electrophoresis on cellulose acetate membranes

Abstract

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