Sequencing and expression of additional xylanase genes from the hyperthermophile Thermotoga maritima FjSS3B.1

Rosalind A. Reeves, Moreland D. Gibbs, Daniel D. Morris, Katherine R. Griffiths, David J. Saul, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Two genes, xynB and xynC, coding for xylanases were isolated from Thermotoga maritima FjSS3B.1 by a genomic-walking-PCR technique. Sequencing of the genes showed that they encode multidomain family 10 xylanases. Only XynB exhibited activity against xylan substrates. The temperature optimum (87°C) and pH optimum (pH 6.5) of XynB are different from the previously reported xylanase, XynA (also a family 10 enzyme), from this organism. The catalytic domain expressed without other domains has a lower temperature optimum, is less thermostable, and has optimal activity at pH 6.5. Despite having a high level of sequence similarity to xynB, xynC appears to be nonfunctional since its encoded protein did not show significant activity on xylan substrates.

Original languageEnglish
Pages (from-to)1532-1537
Number of pages6
JournalApplied and Environmental Microbiology
Volume66
Issue number4
DOIs
Publication statusPublished - Apr 2000

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