SERS driven cross-platform based multiplex pathogen detection

Sandeep P. Ravindranath; Yuling Wang; Joseph Irudayaraj

doi:10.1016/j.snb.2010.12.005

SERS driven cross-platform based multiplex pathogen detection

Sandeep P. Ravindranath, Yuling Wang, Joseph Irudayaraj^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 10² and 10³ CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.

Original language	English
Pages (from-to)	183-190
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	152
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2010.12.005
Publication status	Published - 1 Mar 2011
Externally published	Yes

Keywords

Aptamers
Cross-platform detection
Multiplex detection
Nanotechnology
Pathogens
SERS

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10.1016/j.snb.2010.12.005

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title = "SERS driven cross-platform based multiplex pathogen detection",

abstract = "We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.",

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author = "Ravindranath, \{Sandeep P.\} and Yuling Wang and Joseph Irudayaraj",

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AU - Ravindranath, Sandeep P.

AU - Wang, Yuling

AU - Irudayaraj, Joseph

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AB - We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.

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KW - Multiplex detection

KW - Nanotechnology

KW - Pathogens

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JO - Sensors and Actuators, B: Chemical

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SERS driven cross-platform based multiplex pathogen detection

Abstract

Keywords

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