TY - CHAP
T1 - SERS Nanotags
AU - Lyu, Nana
AU - Wang, Yuling
PY - 2022
Y1 - 2022
N2 - SERS is a vibrational spectral technique, which is widely used in bioanalytical chemistry and life sciences. SERS nanotag as an alternative label for both immunoassay and microscopic bioimaging has experienced tremendous progress in the past few years with unique advantages and applications that are not achievable with organic dyes or molecular fluorophores. The advantages of SERS nanotags include the quantification, sensitivity, photostability, and spectral multiplexing for simultaneous target detection. Significant advances in the rational design and synthesis of bright plasmonic NPs as the core of SERS nanotags have been accomplished, including control over size/shape and nanoassemblies with multiple hot spots. Moreover, the diversity of molecules (from the dye molecules to small molecules) provides a flexible choice of Raman reporters depending on the applications. Along with the development of the protective layers (e.g., polymer/ biopolymer, silica) as well as the spacer/linker molecules, SERS nanotags are expected to find more applications in biomedical and clinical applications. However, the design and synthesis of stable and well-defined 3D plasmonic nanoassemblies with multiple hot spots for very high sensitivity remain as challenging as ever. For reproducibility and quantification, the use of purified colloids with a well-defined composition (no mixtures), as well the design and synthesis of Raman reporters with similar Raman crosssections, is very important for multiplex detection. Furthermore, the stability and storage of SERS nanotags are the other key issues that need to be considered in the future for the reliable application.
AB - SERS is a vibrational spectral technique, which is widely used in bioanalytical chemistry and life sciences. SERS nanotag as an alternative label for both immunoassay and microscopic bioimaging has experienced tremendous progress in the past few years with unique advantages and applications that are not achievable with organic dyes or molecular fluorophores. The advantages of SERS nanotags include the quantification, sensitivity, photostability, and spectral multiplexing for simultaneous target detection. Significant advances in the rational design and synthesis of bright plasmonic NPs as the core of SERS nanotags have been accomplished, including control over size/shape and nanoassemblies with multiple hot spots. Moreover, the diversity of molecules (from the dye molecules to small molecules) provides a flexible choice of Raman reporters depending on the applications. Along with the development of the protective layers (e.g., polymer/ biopolymer, silica) as well as the spacer/linker molecules, SERS nanotags are expected to find more applications in biomedical and clinical applications. However, the design and synthesis of stable and well-defined 3D plasmonic nanoassemblies with multiple hot spots for very high sensitivity remain as challenging as ever. For reproducibility and quantification, the use of purified colloids with a well-defined composition (no mixtures), as well the design and synthesis of Raman reporters with similar Raman crosssections, is very important for multiplex detection. Furthermore, the stability and storage of SERS nanotags are the other key issues that need to be considered in the future for the reliable application.
UR - http://www.scopus.com/inward/record.url?scp=85151118520&partnerID=8YFLogxK
U2 - 10.1142/9789811235252_0006
DO - 10.1142/9789811235252_0006
M3 - Chapter
AN - SCOPUS:85151118520
SN - 9789811235207
T3 - World Scientific Series in Nanoscience and Nanotechnology
SP - 215
EP - 243
BT - World Scientific Reference on Plasmonic Nanomaterials
A2 - Schlücker, Sebastian
PB - World Scientific Publishing
CY - Singapore
ER -