Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR

Kalpani Senevirathna; Thilini Anupama Nanayakkarawasam Mahakapuge; Nadeeka U. Jayawardana; Jayanthe Rajapakse; Chandrika Udumalagala Gamage; Bimalka Seneviratne; Unil Perera; Kehinde Kazeem Kanmodi; Ruwan Jayasinghe

doi:10.1186/s12903-024-04834-1

Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR

Kalpani Senevirathna, Thilini Anupama Nanayakkarawasam Mahakapuge, Nadeeka U. Jayawardana, Jayanthe Rajapakse, Chandrika Udumalagala Gamage, Bimalka Seneviratne, Unil Perera, Kehinde Kazeem Kanmodi^*, Ruwan Jayasinghe

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Oral cancers, which include tumors of the oral cavity, salivary glands, and pharynx, are becoming increasingly prevalent worldwide. Squamous cell carcinoma accounts for over 90% of malignant oral lesions, with oral squamous cell carcinoma (OSCC) being notably common in the Indian subcontinent and other regions of Asia. This is especially true in South-Central Asia, including Sri Lanka, where it is particularly prevalent among men. This study aims to evaluate the levels of Vascular Endothelial Growth Factor-A (VEGF-A) and Cytokeratin-19 (CK-19) mRNAs in whole blood as a potential method for the early detection of OSCC.

Methods: The study included 40 patients (each from OSCC, Oral Submucous Fibrosis (OSF), Oral Leukoplakia (OLK), Oral Lichen Planus (OLP), and 10 healthy controls. The expression levels of VEGF-A and CK-19 mRNAs were measured from extracellular RNA extracted from whole blood samples using real-time reverse transcription polymerase chain reaction (RT-PCR) with sequence-specific primers. Receiver operating characteristic (ROC) curve analysis was used to evaluate the effectiveness of these biomarkers in detecting OSCC.

Results: The results demonstrated a significant increase in blood transcripts of the candidate mRNAs CK-19 and VEGF-A in patients with OSCC, OSF, OLK, and OLP. The Wilcoxon signed-rank test revealed a p-value of 0.002 for each specific comparison between diseased patients and healthy controls (i.e., OSCC vs. HC, OSF vs. HC, OLP vs. HC, OLK vs. HC) for both CK-19 and VEGF-A. When these two biomarkers were used together, they provided a 60% predictive probability for patients with OSCC (p = 0.023).

Conclusion: This study highlights the efficacy of blood mRNA transcriptome diagnostics in detecting OSCC. This innovative clinical approach has the potential to be a robust, efficient, and reliable tool for early cancer detection. Blood-based transcriptomes could be further explored for their effectiveness in various health contexts and for routine health monitoring.

Original language	English
Article number	1062
Pages (from-to)	1-10
Number of pages	10
Journal	BMC Oral Health
Volume	24
Issue number	1
DOIs	https://doi.org/10.1186/s12903-024-04834-1
Publication status	Published - 11 Sept 2024
Externally published	Yes

Bibliographical note

Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Blood
Early detection
mRNA
Oral cancer
Oral potentially malignant disorders
Polymerase chain reaction

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10.1186/s12903-024-04834-1

Publisher version (open access)Final published version, 1.21 MBLicence: CC BY-NC-ND

Cite this

Senevirathna, K., Mahakapuge, T. A. N., Jayawardana, N. U., Rajapakse, J., Gamage, C. U., Seneviratne, B., Perera, U., Kanmodi, K. K., & Jayasinghe, R. (2024). Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR. BMC Oral Health, 24(1), 1-10. Article 1062. https://doi.org/10.1186/s12903-024-04834-1

@article{495baeb8a6d44596bbb76707ba850325,

title = "Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR",

abstract = "Background: Oral cancers, which include tumors of the oral cavity, salivary glands, and pharynx, are becoming increasingly prevalent worldwide. Squamous cell carcinoma accounts for over 90% of malignant oral lesions, with oral squamous cell carcinoma (OSCC) being notably common in the Indian subcontinent and other regions of Asia. This is especially true in South-Central Asia, including Sri Lanka, where it is particularly prevalent among men. This study aims to evaluate the levels of Vascular Endothelial Growth Factor-A (VEGF-A) and Cytokeratin-19 (CK-19) mRNAs in whole blood as a potential method for the early detection of OSCC. Methods: The study included 40 patients (each from OSCC, Oral Submucous Fibrosis (OSF), Oral Leukoplakia (OLK), Oral Lichen Planus (OLP), and 10 healthy controls. The expression levels of VEGF-A and CK-19 mRNAs were measured from extracellular RNA extracted from whole blood samples using real-time reverse transcription polymerase chain reaction (RT-PCR) with sequence-specific primers. Receiver operating characteristic (ROC) curve analysis was used to evaluate the effectiveness of these biomarkers in detecting OSCC. Results: The results demonstrated a significant increase in blood transcripts of the candidate mRNAs CK-19 and VEGF-A in patients with OSCC, OSF, OLK, and OLP. The Wilcoxon signed-rank test revealed a p-value of 0.002 for each specific comparison between diseased patients and healthy controls (i.e., OSCC vs. HC, OSF vs. HC, OLP vs. HC, OLK vs. HC) for both CK-19 and VEGF-A. When these two biomarkers were used together, they provided a 60% predictive probability for patients with OSCC (p = 0.023). Conclusion: This study highlights the efficacy of blood mRNA transcriptome diagnostics in detecting OSCC. This innovative clinical approach has the potential to be a robust, efficient, and reliable tool for early cancer detection. Blood-based transcriptomes could be further explored for their effectiveness in various health contexts and for routine health monitoring.",

keywords = "Blood, Early detection, mRNA, Oral cancer, Oral potentially malignant disorders, Polymerase chain reaction",

author = "Kalpani Senevirathna and Mahakapuge, {Thilini Anupama Nanayakkarawasam} and Jayawardana, {Nadeeka U.} and Jayanthe Rajapakse and Gamage, {Chandrika Udumalagala} and Bimalka Seneviratne and Unil Perera and Kanmodi, {Kehinde Kazeem} and Ruwan Jayasinghe",

note = "Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2024",

month = sep,

day = "11",

doi = "10.1186/s12903-024-04834-1",

language = "English",

volume = "24",

pages = "1--10",

journal = "BMC Oral Health",

issn = "1472-6831",

publisher = "Springer, Springer Nature",

number = "1",

}

Senevirathna, K, Mahakapuge, TAN, Jayawardana, NU, Rajapakse, J, Gamage, CU, Seneviratne, B, Perera, U, Kanmodi, KK & Jayasinghe, R 2024, 'Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR', BMC Oral Health, vol. 24, no. 1, 1062, pp. 1-10. https://doi.org/10.1186/s12903-024-04834-1

Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR. / Senevirathna, Kalpani; Mahakapuge, Thilini Anupama Nanayakkarawasam; Jayawardana, Nadeeka U. et al.
In: BMC Oral Health, Vol. 24, No. 1, 1062, 11.09.2024, p. 1-10.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR

AU - Senevirathna, Kalpani

AU - Mahakapuge, Thilini Anupama Nanayakkarawasam

AU - Jayawardana, Nadeeka U.

AU - Rajapakse, Jayanthe

AU - Gamage, Chandrika Udumalagala

AU - Seneviratne, Bimalka

AU - Perera, Unil

AU - Kanmodi, Kehinde Kazeem

AU - Jayasinghe, Ruwan

N1 - Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2024/9/11

Y1 - 2024/9/11

N2 - Background: Oral cancers, which include tumors of the oral cavity, salivary glands, and pharynx, are becoming increasingly prevalent worldwide. Squamous cell carcinoma accounts for over 90% of malignant oral lesions, with oral squamous cell carcinoma (OSCC) being notably common in the Indian subcontinent and other regions of Asia. This is especially true in South-Central Asia, including Sri Lanka, where it is particularly prevalent among men. This study aims to evaluate the levels of Vascular Endothelial Growth Factor-A (VEGF-A) and Cytokeratin-19 (CK-19) mRNAs in whole blood as a potential method for the early detection of OSCC. Methods: The study included 40 patients (each from OSCC, Oral Submucous Fibrosis (OSF), Oral Leukoplakia (OLK), Oral Lichen Planus (OLP), and 10 healthy controls. The expression levels of VEGF-A and CK-19 mRNAs were measured from extracellular RNA extracted from whole blood samples using real-time reverse transcription polymerase chain reaction (RT-PCR) with sequence-specific primers. Receiver operating characteristic (ROC) curve analysis was used to evaluate the effectiveness of these biomarkers in detecting OSCC. Results: The results demonstrated a significant increase in blood transcripts of the candidate mRNAs CK-19 and VEGF-A in patients with OSCC, OSF, OLK, and OLP. The Wilcoxon signed-rank test revealed a p-value of 0.002 for each specific comparison between diseased patients and healthy controls (i.e., OSCC vs. HC, OSF vs. HC, OLP vs. HC, OLK vs. HC) for both CK-19 and VEGF-A. When these two biomarkers were used together, they provided a 60% predictive probability for patients with OSCC (p = 0.023). Conclusion: This study highlights the efficacy of blood mRNA transcriptome diagnostics in detecting OSCC. This innovative clinical approach has the potential to be a robust, efficient, and reliable tool for early cancer detection. Blood-based transcriptomes could be further explored for their effectiveness in various health contexts and for routine health monitoring.

AB - Background: Oral cancers, which include tumors of the oral cavity, salivary glands, and pharynx, are becoming increasingly prevalent worldwide. Squamous cell carcinoma accounts for over 90% of malignant oral lesions, with oral squamous cell carcinoma (OSCC) being notably common in the Indian subcontinent and other regions of Asia. This is especially true in South-Central Asia, including Sri Lanka, where it is particularly prevalent among men. This study aims to evaluate the levels of Vascular Endothelial Growth Factor-A (VEGF-A) and Cytokeratin-19 (CK-19) mRNAs in whole blood as a potential method for the early detection of OSCC. Methods: The study included 40 patients (each from OSCC, Oral Submucous Fibrosis (OSF), Oral Leukoplakia (OLK), Oral Lichen Planus (OLP), and 10 healthy controls. The expression levels of VEGF-A and CK-19 mRNAs were measured from extracellular RNA extracted from whole blood samples using real-time reverse transcription polymerase chain reaction (RT-PCR) with sequence-specific primers. Receiver operating characteristic (ROC) curve analysis was used to evaluate the effectiveness of these biomarkers in detecting OSCC. Results: The results demonstrated a significant increase in blood transcripts of the candidate mRNAs CK-19 and VEGF-A in patients with OSCC, OSF, OLK, and OLP. The Wilcoxon signed-rank test revealed a p-value of 0.002 for each specific comparison between diseased patients and healthy controls (i.e., OSCC vs. HC, OSF vs. HC, OLP vs. HC, OLK vs. HC) for both CK-19 and VEGF-A. When these two biomarkers were used together, they provided a 60% predictive probability for patients with OSCC (p = 0.023). Conclusion: This study highlights the efficacy of blood mRNA transcriptome diagnostics in detecting OSCC. This innovative clinical approach has the potential to be a robust, efficient, and reliable tool for early cancer detection. Blood-based transcriptomes could be further explored for their effectiveness in various health contexts and for routine health monitoring.

KW - Blood

KW - Early detection

KW - mRNA

KW - Oral cancer

KW - Oral potentially malignant disorders

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=85203546243&partnerID=8YFLogxK

U2 - 10.1186/s12903-024-04834-1

DO - 10.1186/s12903-024-04834-1

M3 - Article

C2 - 39261828

AN - SCOPUS:85203546243

SN - 1472-6831

VL - 24

SP - 1

EP - 10

JO - BMC Oral Health

JF - BMC Oral Health

IS - 1

M1 - 1062

ER -

Serum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR

Abstract

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Keywords

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