Serum profiling for identification of autoantibody signatures in diseases using protein microarrays

Shabarni Gupta, K. P. Manubhai, Shuvolina Mukherjee, Sanjeeva Srivastava*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Protein microarrays are platforms for studying protein-protein interactions and identifying disease-related self-antigens/autoantigens, which elicit an immune response in a high-throughput format. Protein arrays have been extensively used over the past two decades for several clinical applications. By using this platform, serum containing autoantibodies against potential self-antigens can be screened on proteome-wide arrays, harboring a large repertoire of full-length human proteins. Identification of such autoantigens can help deducing early diagnostic, as well as, prognostic markers in case of malignancies, autoimmune disorders, and other systemic diseases. Here, we provide an overview of the protein microarray technology along with details of an established method to study autoantibody profiles from patient sera.

Original languageEnglish
Title of host publicationSerum/ plasma proteomics
Subtitle of host publicationmethods and protocols
EditorsDavid W. Greening, Richard J. Simpson
Place of PublicationNew York
PublisherHumana Press Inc.
Pages303-315
Number of pages13
Edition2nd
ISBN (Electronic)9781493970575
ISBN (Print)9781493970568
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1619
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • autoantibody
  • biomarker discovery
  • diagnostics
  • protein microarrays
  • serum profiling

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