TY - JOUR
T1 - Serum proteome analysis of vivax malaria
T2 - An insight into the disease pathogenesis and host immune response
AU - Ray, Sandipan
AU - Kamath, Karthik S.
AU - Srivastava, Rajneesh
AU - Raghu, Dinesh
AU - Gollapalli, Kishore
AU - Jain, Rekha
AU - Gupta, Shipra V.
AU - Ray, Sayantan
AU - Taur, Santosh
AU - Dhali, Snigdha
AU - Gogtay, Nithya
AU - Thatte, Urmila
AU - Srikanth, Rapole
AU - Patankar, Swati
AU - Srivastava, Sanjeeva
PY - 2012/6/6
Y1 - 2012/6/6
N2 - Vivax malaria is the most widely distributed human malaria resulting in 80-300. million clinical cases every year. It causes severe infection and mortality but is generally regarded as a benign disease and has not been investigated in detail. The present study aimed to perform human serum proteome analysis in a malaria endemic area in India to identify potential serum biomarkers for vivax malaria and understand host response. The proteomic analysis was performed on 16 age and gender matched subjects (vivax patients and control) in duplicate. Protein extraction protocols were optimized for large coverage of the serum proteome and to obtain high-resolution data. Identification of 67 differentially expressed and statistically significant (Student's t-test; p<0.05) protein spots was established by MALDI-TOF/TOF mass spectrometry. Many of the identified proteins such as apolipoprotein A and E, serum amyloid A and P, haptoglobin, ceruloplasmin, and hemopexin are interesting from a diagnostic point of view and could further be studied as potential serum biomarkers. The differentially expressed serum proteins in vivax malaria identified in this study were subjected to functional pathway analysis using multiple software, including Ingenuity Pathway Analysis (IPA), Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) functional annotation tool for better understanding of the biological context of the identified proteins, their involvement in various physiological pathways and association with disease pathogenesis. Functional pathway analysis of the differentially expressed proteins suggested the modulation of multiple vital physiological pathways, including acute phase response signaling, complement and coagulation cascades, hemostasis and vitamin D metabolism pathway due to this parasitic infection. This article is part of a Special Issue entitled: Proteomics: The clinical link.
AB - Vivax malaria is the most widely distributed human malaria resulting in 80-300. million clinical cases every year. It causes severe infection and mortality but is generally regarded as a benign disease and has not been investigated in detail. The present study aimed to perform human serum proteome analysis in a malaria endemic area in India to identify potential serum biomarkers for vivax malaria and understand host response. The proteomic analysis was performed on 16 age and gender matched subjects (vivax patients and control) in duplicate. Protein extraction protocols were optimized for large coverage of the serum proteome and to obtain high-resolution data. Identification of 67 differentially expressed and statistically significant (Student's t-test; p<0.05) protein spots was established by MALDI-TOF/TOF mass spectrometry. Many of the identified proteins such as apolipoprotein A and E, serum amyloid A and P, haptoglobin, ceruloplasmin, and hemopexin are interesting from a diagnostic point of view and could further be studied as potential serum biomarkers. The differentially expressed serum proteins in vivax malaria identified in this study were subjected to functional pathway analysis using multiple software, including Ingenuity Pathway Analysis (IPA), Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) functional annotation tool for better understanding of the biological context of the identified proteins, their involvement in various physiological pathways and association with disease pathogenesis. Functional pathway analysis of the differentially expressed proteins suggested the modulation of multiple vital physiological pathways, including acute phase response signaling, complement and coagulation cascades, hemostasis and vitamin D metabolism pathway due to this parasitic infection. This article is part of a Special Issue entitled: Proteomics: The clinical link.
KW - Disease pathogenesis
KW - Immune response
KW - Plasmodium
KW - Proteomics
KW - Serum
KW - Vivax malaria
UR - http://www.scopus.com/inward/record.url?scp=84861347645&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2011.10.018
DO - 10.1016/j.jprot.2011.10.018
M3 - Article
C2 - 22086083
AN - SCOPUS:84861347645
SN - 1874-3919
VL - 75
SP - 3063
EP - 3080
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 10
ER -