TY - JOUR
T1 - Shotgun proteomic analysis of photoperiod regulated dormancy induction in grapevine
AU - George, Iniga S.
AU - Fennell, Anne Y.
AU - Haynes, Paul A.
PY - 2018/9/15
Y1 - 2018/9/15
N2 - Certain grapevine genotypes become dormant in response to decreasing photoperiod and others require low temperature or both environmental cues to induce dormancy. This study used a proteomic approach to gain an understanding of the underlying molecular events involved in bud dormancy commitment. Two F2 siblings (F2-110 and F2-040) with differences in photoperiod induced dormancy responsiveness were subjected to long day (LD, 15 h, paradormancy maintenance or dormancy inhibition) or short day (SD, 13 h, endodormancy commitment) treatment. Proteins were extracted at two time points (28 days and 42 days) of LD and SD photoperiod exposure, and label-free quantitative shotgun proteomic analysis was performed for three biological replicates of each treatment and time point. A total of 1577 non-redundant proteins were identified in the combined dataset of eight different conditions (2 genotypes, 2 photoperiods and 2 timepoints, available via ProteomeXchange with identifier PXD001627). Genotype specific patterns of budbreak and protein expression were detected in response to the differential photoperiod treatment at the two time points. Peroxidases, dehydrogenases and superoxide dismutases were more abundant at 42 SD than at 28 SD in the dormancy responsive F2-110, suggesting that oxidative stress response related proteins could be markers of endodormancy commitment in grapevine buds.
AB - Certain grapevine genotypes become dormant in response to decreasing photoperiod and others require low temperature or both environmental cues to induce dormancy. This study used a proteomic approach to gain an understanding of the underlying molecular events involved in bud dormancy commitment. Two F2 siblings (F2-110 and F2-040) with differences in photoperiod induced dormancy responsiveness were subjected to long day (LD, 15 h, paradormancy maintenance or dormancy inhibition) or short day (SD, 13 h, endodormancy commitment) treatment. Proteins were extracted at two time points (28 days and 42 days) of LD and SD photoperiod exposure, and label-free quantitative shotgun proteomic analysis was performed for three biological replicates of each treatment and time point. A total of 1577 non-redundant proteins were identified in the combined dataset of eight different conditions (2 genotypes, 2 photoperiods and 2 timepoints, available via ProteomeXchange with identifier PXD001627). Genotype specific patterns of budbreak and protein expression were detected in response to the differential photoperiod treatment at the two time points. Peroxidases, dehydrogenases and superoxide dismutases were more abundant at 42 SD than at 28 SD in the dormancy responsive F2-110, suggesting that oxidative stress response related proteins could be markers of endodormancy commitment in grapevine buds.
KW - Vitis
KW - Grape
KW - Photoperiod
KW - Quantitative proteomics
KW - Dormancy
KW - Daylength
UR - http://www.scopus.com/inward/record.url?scp=85048527736&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/IC13010009
U2 - 10.1016/j.jprot.2018.05.014
DO - 10.1016/j.jprot.2018.05.014
M3 - Article
C2 - 29857064
AN - SCOPUS:85048527736
SN - 1874-3919
VL - 187
SP - 13
EP - 24
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -