Shotgun proteomics of coelomic fluid from the purple sea urchin, Strongylocentrotus purpuratus

Nolwenn M. Dheilly*, David A. Raftos, Paul A. Haynes, L. Courtney Smith, Sham V. Nair

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The purple sea urchin has a complex immune system that is likely mediated by gene expression in coelomocytes (blood cells). A broad array of potential immune receptors and immune response proteins has been deduced from their gene models. Here we use shotgun mass spectrometry to describe 307 proteins with possible immune function in sea urchins including proteins involved in the complement pathway and numerous SRCRs. The relative abundance of dual oxidase 1, ceruloplasmin, ferritin and transferrin suggests the production of reactive oxygen species in coelomocytes and the sequestration of iron. Proteins such as selectin, cadherin, talin, galectin, amassin and the Von Willebrand factor may be involved in generating a strong clotting reaction. Cell signaling proteins include a guanine nucleotide binding protein, the Rho GDP dissociation factor, calcium storage molecules and a variety of lipoproteins. However, based on this dataset, the expression of TLRs, NLRs and fibrinogen domain containing proteins in coelomic fluid and coelomocytes could not be verified.

Original languageEnglish
Pages (from-to)35-50
Number of pages16
JournalDevelopmental and Comparative Immunology
Volume40
Issue number1
DOIs
Publication statusPublished - May 2013

Bibliographical note

A corrigendum for this article exists in Developmental & Comparative Immunology, vol. 40, issue 3-4, p. 325. DOI: 10.1016/j.dci.2013.05.015

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