Signal generation at an electrochemical immunosensor via the direct oxidation of an electroactive label

M Akram, MC Stuart, Danny Wong

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this paper, we report the development of an electrochemical immunosensor with a direct signal generation scheme. To achieve this, ferrocenecarboxylic acid was conjugated as an electroactive label to an immunocomplex immobilized on an electrochemically pretreated glassy carbon electrode. The pregnancy hormone and tumor marker. human chorionic gonadotrophin (hCG), was employed as a model analyte. Direct oxidation of ferrocenecarboxylic acid facilitated a signal generation scheme for the quantification of hCG at the immunosensor. Evaluation of the immunosensor performance has yielded statistically acceptable agreement in hCG levels present in several human serum samples compared to those reported by a Sydney hospital.

Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalElectroanalysis
Volume18
Issue number3
DOIs
Publication statusPublished - Feb 2006

Keywords

  • electrochemical immunosensors
  • hCG detection
  • ferrocenecarboxylic acid
  • direct signal-generation scheme
  • non-competitive immunoassays
  • SURFACE-PLASMON RESONANCE
  • SELF-ASSEMBLED MONOLAYERS
  • GLASSY-CARBON ELECTRODE
  • HUMAN CHORIONIC-GONADOTROPIN
  • CONJUGATED IMMUNOGLOBULIN-G
  • HORSERADISH-PEROXIDASE
  • FLOW IMMUNOASSAY
  • AMPEROMETRIC IMMUNOSENSOR
  • TROPHOBLASTIC DISEASE
  • THIOCTIC ACID

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