Silver nanoparticles enhance Pseudomonas aeruginosa PAO1 biofilm detachment

Ching-Yee Loo, Paul M. Young, Rosalia Cavaliere, Cynthia B. Whitchurch, Wing-Hin Lee, Ramin Rohanizadeh

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Objectives: Silver nanoparticles (AgNPs) with a size ranging from 7 to 70 nm were synthesized using the ascorbic acid-citrate seed-mediated growth approach at room temperature.

Methods: The 8 nm silver particles were prepared using gallic acid in alkaline conditions and used as seed to prepare AgNPs.

Results: The presence of ascorbic acid and citrate allows the regulation of size and size distribution of the nanoparticles. The increase in free silver ion-to-seed ratio (Ag+/Ag0) resulted in changes of particle shape from spherical to pseudo-spherical and minor cylindrical shape. Further, a repetitive seeding approach resulted in the formation of pseudo-spherical particles with higher polydispersity index and minor distributions of tetrahedral particles. Citrate-capped AgNPs were stable and did not agglomerate upon centrifugation. The effect of AgNPs on biofilm reduction was evaluated using static culture on 96-well microtiter plates. Results showed that AgNPs with the smallest average diameter were most effective in the reduction of Pseudomonas aeruginosa biofilm colonies, which accounted for 90% of removal.

Conclusion: The biofilm removal activities of the nanoparticles were found to be concentration-independent particularly for the concentration within the range of 80–200 µg/mL.
Original languageEnglish
Pages (from-to)719-729
Number of pages11
JournalDrug Development and Industrial Pharmacy
Issue number6
Publication statusPublished - 2014
Externally publishedYes


  • Antibacterial
  • biofilm
  • chemical reduction
  • Pseudomonas aeruginosa
  • silver nanoparticles


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