Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets

Ailing Su; Yuan Liu; Weihan Sun; Chongyang Liang; Weiqing Xu; Alison Rodger; Jim Piper; Yuling Wang; Shuping Xu

doi:10.1021/acsanm.4c01925

Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets

Ailing Su, Yuan Liu, Weihan Sun, Chongyang Liang, Weiqing Xu, Alison Rodger, Jim Piper, Yuling Wang^*, Shuping Xu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.

Original language	English
Pages (from-to)	9800-9808
Number of pages	9
Journal	ACS Applied Nano Materials
Volume	7
Issue number	8
Early online date	13 Apr 2024
DOIs	https://doi.org/10.1021/acsanm.4c01925
Publication status	Published - 26 Apr 2024

Keywords

CRISPR/dCas9
point mutation detection
SERS nanotag
KRAS G12C
KRAS G12V

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10.1021/acsanm.4c01925

Cite this

@article{fb11234df9a040ef8b859494c22e5389,

title = "Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets",

abstract = "Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.",

keywords = "CRISPR/dCas9, point mutation detection, SERS nanotag, KRAS G12C, KRAS G12V",

author = "Ailing Su and Yuan Liu and Weihan Sun and Chongyang Liang and Weiqing Xu and Alison Rodger and Jim Piper and Yuling Wang and Shuping Xu",

year = "2024",

month = apr,

day = "26",

doi = "10.1021/acsanm.4c01925",

language = "English",

volume = "7",

pages = "9800--9808",

journal = "ACS Applied Nano Materials",

issn = "2574-0970",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets

AU - Su, Ailing

AU - Liu, Yuan

AU - Sun, Weihan

AU - Liang, Chongyang

AU - Xu, Weiqing

AU - Rodger, Alison

AU - Piper, Jim

AU - Wang, Yuling

AU - Xu, Shuping

PY - 2024/4/26

Y1 - 2024/4/26

N2 - Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.

AB - Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.

KW - CRISPR/dCas9

KW - point mutation detection

KW - SERS nanotag

KW - KRAS G12C

KW - KRAS G12V

UR - https://pubs.acs.org/doi/10.1021/acsanm.4c01925

UR - http://purl.org/au-research/grants/arc/CE140100003

UR - https://purl.org/au-research/grants/arc/IC210100040

UR - https://www.scopus.com/pages/publications/85190741568

U2 - 10.1021/acsanm.4c01925

DO - 10.1021/acsanm.4c01925

M3 - Article

SN - 2574-0970

VL - 7

SP - 9800

EP - 9808

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

IS - 8

ER -

Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets

Abstract

Keywords

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FAAB: ARC Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB)

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Silver nanoparticles with dual-recognition via CRISPR/dCas9 for SERS identification of two KRAS mutations in nucleic acid targets

Abstract

Keywords

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FAAB: ARC Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB)

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