TY - GEN
T1 - Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging
AU - Deng, Wei
AU - Drozdowicz-Tomsia, Krystyna
AU - Jin, Dayong
AU - Goldys, Ewa M.
PY - 2010
Y1 - 2010
N2 - While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.
AB - While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.
UR - http://www.scopus.com/inward/record.url?scp=80555157617&partnerID=8YFLogxK
U2 - 10.1109/ICONN.2010.6045197
DO - 10.1109/ICONN.2010.6045197
M3 - Conference proceeding contribution
AN - SCOPUS:80555157617
SN - 9781424452613
T3 - International Conference on Nanoscience and Nanotechnology: Proceedings
SP - 108
EP - 111
BT - 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010)
A2 - Dzurak, Andrew
PB - Institute of Electrical and Electronics Engineers (IEEE)
CY - Pistcataway, NJ
T2 - 2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010
Y2 - 22 February 2010 through 26 February 2010
ER -