Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging

Wei Deng*, Krystyna Drozdowicz-Tomsia, Dayong Jin, Ewa M. Goldys

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.

Original languageEnglish
Title of host publication2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010)
EditorsAndrew Dzurak
Place of PublicationPistcataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages108-111
Number of pages4
ISBN (Electronic)9781424452620, 9781424452620
ISBN (Print)9781424452613
DOIs
Publication statusPublished - 2010
Event2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010 - Sydney, Australia
Duration: 22 Feb 201026 Feb 2010

Publication series

NameInternational Conference on Nanoscience and Nanotechnology: Proceedings
PublisherIEEE
ISSN (Print)2150-3591
ISSN (Electronic)2150-3605

Other

Other2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010
CountryAustralia
CitySydney
Period22/02/1026/02/10

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