Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging

Wei Deng, Krystyna Drozdowicz-Tomsia, Dayong Jin, Ewa M. Goldys

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

Abstract

While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.

LanguageEnglish
Title of host publication2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010)
EditorsAndrew Dzurak
Place of PublicationPistcataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages108-111
Number of pages4
ISBN (Electronic)9781424452620, 9781424452620
ISBN (Print)9781424452613
DOIs
Publication statusPublished - 2010
Event2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010 - Sydney, Australia
Duration: 22 Feb 201026 Feb 2010

Publication series

NameInternational Conference on Nanoscience and Nanotechnology: Proceedings
PublisherIEEE
ISSN (Print)2150-3591
ISSN (Electronic)2150-3605

Other

Other2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010
CountryAustralia
CitySydney
Period22/02/1026/02/10

Fingerprint

Nanostructures
Immunoassay
Silver
Fluorescence
Silicon Dioxide
Silica
Flow cytometry
Flow Cytometry
Metals
Europium
Fluorophores
Bioassay
Biological Assay
Glass
Immunoglobulin G
Observation
Imaging techniques
Substrates

Cite this

Deng, W., Drozdowicz-Tomsia, K., Jin, D., & Goldys, E. M. (2010). Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging. In A. Dzurak (Ed.), 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010) (pp. 108-111). [6045197] (International Conference on Nanoscience and Nanotechnology: Proceedings). Pistcataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ICONN.2010.6045197
Deng, Wei ; Drozdowicz-Tomsia, Krystyna ; Jin, Dayong ; Goldys, Ewa M. / Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging. 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010). editor / Andrew Dzurak. Pistcataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 2010. pp. 108-111 (International Conference on Nanoscience and Nanotechnology: Proceedings).
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abstract = "While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.",
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Deng, W, Drozdowicz-Tomsia, K, Jin, D & Goldys, EM 2010, Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging. in A Dzurak (ed.), 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010)., 6045197, International Conference on Nanoscience and Nanotechnology: Proceedings, Institute of Electrical and Electronics Engineers (IEEE), Pistcataway, NJ, pp. 108-111, 2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN - 2010, Sydney, Australia, 22/02/10. https://doi.org/10.1109/ICONN.2010.6045197

Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging. / Deng, Wei; Drozdowicz-Tomsia, Krystyna; Jin, Dayong; Goldys, Ewa M.

2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010). ed. / Andrew Dzurak. Pistcataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 2010. p. 108-111 6045197 (International Conference on Nanoscience and Nanotechnology: Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

TY - GEN

T1 - Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging

AU - Deng, Wei

AU - Drozdowicz-Tomsia, Krystyna

AU - Jin, Dayong

AU - Goldys, Ewa M.

PY - 2010

Y1 - 2010

N2 - While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.

AB - While the principle of fluorescence enhancement of metal nanostructures is well known, the utility of this effect in practical methodologies used in analytical laboratories remains to be established. In this work we explored the advantage of fluorescence enhancement for flow cytometry and bioimaging. We reported the observation of metal-enhanced fluorescence emission of fluorophores located on the surface of silver nanostructure-coated silica beads, suitable for flow cytometry detection. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay. Approximately 8.5-fold higher fluorescence intensities at 430 nm excitation was observed from silvered 400 nm silica beads deposited on glass as compared to the control sample. Enhanced fluorescence signals, by a factor of 2, were also observed in imaging of cells labeled with the europium chelate, 4,4'-bis(1,1,1,2,2,3,3-heptafluoro-4,6- hexanedion-6-yl)chlorosulfo-o-terphenyl-Eu3 (BHHCT-Eu3), when such silver-coated silica beads were used as substrates. These results indicated that silver nanostructures-coated silica beads are able to provide intensified fluorescence signals for bioassays and bioimaging.

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U2 - 10.1109/ICONN.2010.6045197

DO - 10.1109/ICONN.2010.6045197

M3 - Conference proceeding contribution

SN - 9781424452613

T3 - International Conference on Nanoscience and Nanotechnology: Proceedings

SP - 108

EP - 111

BT - 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010)

A2 - Dzurak, Andrew

PB - Institute of Electrical and Electronics Engineers (IEEE)

CY - Pistcataway, NJ

ER -

Deng W, Drozdowicz-Tomsia K, Jin D, Goldys EM. Silver nanostructure coated beads enhance fluorescence for sensitive immunoassays and bioimaging. In Dzurak A, editor, 2010 International Conference on Nanoscience and Nanotechnology (ICONN 2010). Pistcataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). 2010. p. 108-111. 6045197. (International Conference on Nanoscience and Nanotechnology: Proceedings). https://doi.org/10.1109/ICONN.2010.6045197