Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Kyung-Min Lee; Arup Neogi; Purnima Basu Neogi; Minjung Kim; Bongsoo Kim; Rafal Luchowski; Zygmunt Gryczynski; Nils Calander; Tae-Youl Choi

doi:10.1117/1.3579157

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Kyung-Min Lee, Arup Neogi, Purnima Basu Neogi, Minjung Kim, Bongsoo Kim, Rafal Luchowski, Zygmunt Gryczynski, Nils Calander, Tae-Youl Choi

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Abstract

We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

Original language	English
Pages (from-to)	056008-1-056008-5
Number of pages	5
Journal	Journal of Biomedical Optics
Volume	16
Issue number	5
DOIs	https://doi.org/10.1117/1.3579157
Publication status	Published - 2011

Bibliographical note

Copyright 2011 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

Ag nanowire
Cell imaging
Fluorescence
Focused ion beam
Nanodot

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10.1117/1.3579157

Publisher Version (open access)Final published version, 591 KBLicence: Unspecified

Cite this

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title = "Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging",

abstract = "We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.",

keywords = "Ag nanowire, Cell imaging, Fluorescence, Focused ion beam, Nanodot",

author = "Kyung-Min Lee and Arup Neogi and Neogi, {Purnima Basu} and Minjung Kim and Bongsoo Kim and Rafal Luchowski and Zygmunt Gryczynski and Nils Calander and Tae-Youl Choi",

note = "Copyright 2011 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.",

year = "2011",

doi = "10.1117/1.3579157",

language = "English",

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pages = "056008--1--056008--5",

journal = "Journal of Biomedical Optics",

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TY - JOUR

T1 - Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

AU - Lee, Kyung-Min

AU - Neogi, Arup

AU - Neogi, Purnima Basu

AU - Kim, Minjung

AU - Kim, Bongsoo

AU - Luchowski, Rafal

AU - Gryczynski, Zygmunt

AU - Calander, Nils

AU - Choi, Tae-Youl

N1 - Copyright 2011 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2011

Y1 - 2011

N2 - We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

AB - We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

KW - Ag nanowire

KW - Cell imaging

KW - Fluorescence

KW - Focused ion beam

KW - Nanodot

U2 - 10.1117/1.3579157

DO - 10.1117/1.3579157

M3 - Article

C2 - 21639576

SN - 1083-3668

VL - 16

SP - 056008-1-056008-5

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 5

ER -

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Abstract

Bibliographical note

Keywords

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