Simple bead assay for detection of live bacteria (Escherichia coli)

Philip Butterworth, Henrique T M C M Baltar, Martin Kratzmeier, Ewa M. Goldys

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Bead assays are an important rapid microbial detection technology suitable for extremely low pathogen levels. We report a bead assay for rRNA extracted from Escherichia coli K12 that does not require amplification steps and has readout on an Agilent 2100 Bioanalyzer flow cytometry system. Our assay was able to detect 125 ng of RNA, which is 16 times less than reported earlier. The specificity was extremely high, with no binding to a negative control organism (Bacillus subtilis). We discuss challenges faced during optimization of the key assay components, such as varying amounts of RNA in the samples, number of beads, aggregation, and reproducibility.

Original languageEnglish
Pages (from-to)1443-1447
Number of pages5
JournalAnalytical Chemistry
Volume83
Issue number4
DOIs
Publication statusPublished - 15 Feb 2011

Fingerprint

Dive into the research topics of 'Simple bead assay for detection of live bacteria (Escherichia coli)'. Together they form a unique fingerprint.

Cite this