Simultaneous concentration and separation of proteins in a nanochannel

D. W. Inglis*, N. Calander, E. M. Goldys

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

A nanofluidic device capable of simultaneous concentration and separation of proteins is described. The device is fabricated by standard microlithography in silicon and glass and uses concentration polarization effects that are known to arise in nanochannels to trap proteins based on electrophoretic mobility. The work is supported by COMSOL based FEM simulations of ions, electric potential and fluid flow that predict strong enhancement with the capacity for separation. Most other work in this field has described trapping on the micro side of a micro-nano system where fluid volumes are larger, but the capacity for separation is limited. This paper describes trapping with and without an imposed salt gradient.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Place of PublicationHoboken, New Jersey
PublisherJohn Wiley & Sons
Pages2068-2070
Number of pages3
Volume50
ISBN (Print)9781618390622
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period3/10/107/10/10

Keywords

  • Concentration polarization
  • Electropreconcentration
  • Nanofluidics
  • Preconcentration
  • Protein separation

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