Abstract
A nanofluidic device capable of simultaneous concentration and separation of proteins is described. The device is fabricated by standard microlithography in silicon and glass and uses concentration polarization effects that are known to arise in nanochannels to trap proteins based on electrophoretic mobility. The work is supported by COMSOL based FEM simulations of ions, electric potential and fluid flow that predict strong enhancement with the capacity for separation. Most other work in this field has described trapping on the micro side of a micro-nano system where fluid volumes are larger, but the capacity for separation is limited. This paper describes trapping with and without an imposed salt gradient.
| Original language | English |
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| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
| Place of Publication | Hoboken, New Jersey |
| Publisher | John Wiley & Sons |
| Pages | 2068-2070 |
| Number of pages | 3 |
| Volume | 50 |
| ISBN (Print) | 9781618390622 |
| Publication status | Published - 2010 |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 |
Other
| Other | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Country | Netherlands |
| City | Groningen |
| Period | 3/10/10 → 7/10/10 |
Keywords
- Concentration polarization
- Electropreconcentration
- Nanofluidics
- Preconcentration
- Protein separation