Sodium chloride fixation of tissues under field conditions in tropical countries

Waldemar L. Olszewski*, Dorota Zolich, Gurusami Manokaran, Mani F. Tripathi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Fixation of skin and lymph node fragments in anhydric sodium chloride at room temperature for periods of weeks or months was found to preserve morphological structures and immunoreactivity. Fragments of a size of 6-8×6×6 mm were dried and placed in anhydric sodium chloride powder in sealed containers. Prior to further processing, the specimens were desalinated and rehydrated, then snap-frozen, sectioned, fixed in acetone and stained with monoclonal antibodies. The intensity of staining of molecular structures did not differ from that of primarily snap-frozen specimens. The method allows fixation of tissues under field conditions in tropical countries and at places lacking freezing facilities.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of Immunological Methods
Volume284
Issue number1-2
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Keywords

  • Fixation
  • Immunohistochemistry
  • Lymph node
  • Skin
  • Sodium chloride

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