Fixation of skin and lymph node fragments in anhydric sodium chloride at room temperature for periods of weeks or months was found to preserve morphological structures and immunoreactivity. Fragments of a size of 6-8×6×6 mm were dried and placed in anhydric sodium chloride powder in sealed containers. Prior to further processing, the specimens were desalinated and rehydrated, then snap-frozen, sectioned, fixed in acetone and stained with monoclonal antibodies. The intensity of staining of molecular structures did not differ from that of primarily snap-frozen specimens. The method allows fixation of tissues under field conditions in tropical countries and at places lacking freezing facilities.
- Lymph node
- Sodium chloride