TY - JOUR
T1 - Sodium chloride fixation of tissues under field conditions in tropical countries
AU - Olszewski, Waldemar L.
AU - Zolich, Dorota
AU - Manokaran, Gurusami
AU - Tripathi, Mani F.
PY - 2004/1
Y1 - 2004/1
N2 - Fixation of skin and lymph node fragments in anhydric sodium chloride at room temperature for periods of weeks or months was found to preserve morphological structures and immunoreactivity. Fragments of a size of 6-8×6×6 mm were dried and placed in anhydric sodium chloride powder in sealed containers. Prior to further processing, the specimens were desalinated and rehydrated, then snap-frozen, sectioned, fixed in acetone and stained with monoclonal antibodies. The intensity of staining of molecular structures did not differ from that of primarily snap-frozen specimens. The method allows fixation of tissues under field conditions in tropical countries and at places lacking freezing facilities.
AB - Fixation of skin and lymph node fragments in anhydric sodium chloride at room temperature for periods of weeks or months was found to preserve morphological structures and immunoreactivity. Fragments of a size of 6-8×6×6 mm were dried and placed in anhydric sodium chloride powder in sealed containers. Prior to further processing, the specimens were desalinated and rehydrated, then snap-frozen, sectioned, fixed in acetone and stained with monoclonal antibodies. The intensity of staining of molecular structures did not differ from that of primarily snap-frozen specimens. The method allows fixation of tissues under field conditions in tropical countries and at places lacking freezing facilities.
KW - Fixation
KW - Immunohistochemistry
KW - Lymph node
KW - Skin
KW - Sodium chloride
UR - http://www.scopus.com/inward/record.url?scp=0347634543&partnerID=8YFLogxK
U2 - 10.1016/j.jim.2003.09.019
DO - 10.1016/j.jim.2003.09.019
M3 - Article
C2 - 14736415
AN - SCOPUS:0347634543
SN - 0022-1759
VL - 284
SP - 39
EP - 44
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -