Solid-binding peptides: immobilisation strategies for extremophile biocatalysis in biotechnology

Anwar Sunna, Andrew Care, PL Bergquist

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Solid-binding peptides (SBP’s) have the ability to bind with high affinity and selectivity to the surfaces of a diverse range of solid materials. They offer a simple and versatile method for the directed immobilisation and orientation of proteins and enzymes onto solid supports without impeding their catalytic functionality. This chapter describes SBPs and their potential applications in the isolation, purification and reuse of thermostable enzymes using readily-available and inexpensive silica-based matrices. We suggest some prospects for the introduction of thermostable enzymes immobilised onto solid matrices using SBPs into several of areas of applied biotechnology. In particular, we outline conceptual applications for immobilised enzymes in cell-free synthetic biology, biofuels production and in gas phase biocatalysis for the capture of carbon dioxide.
LanguageEnglish
Title of host publicationBiotechnology of Extremophiles
Subtitle of host publicationAdvances and challenges
EditorsPabulo H. Rampelotto
Place of PublicationCham, Switzerland
PublisherSpringer, Springer Nature
Pages637-674
Number of pages38
ISBN (Electronic)9783319135212
ISBN (Print)9783319135205
DOIs
Publication statusPublished - 2016

Publication series

NameGrand Challenges in Biology and Biotechnology
PublisherSpringer International Publishing
Volume1
ISSN (Electronic)2367-1017

Fingerprint

Biotechnology
Immobilized Enzymes
Peptides
Biofuels
Enzymes
Carbon Dioxide
Silicon Dioxide
Purification
Gases
Biocatalysis
Proteins

Cite this

Sunna, A., Care, A., & Bergquist, PL. (2016). Solid-binding peptides: immobilisation strategies for extremophile biocatalysis in biotechnology. In P. H. Rampelotto (Ed.), Biotechnology of Extremophiles: Advances and challenges (pp. 637-674). (Grand Challenges in Biology and Biotechnology; Vol. 1). Cham, Switzerland: Springer, Springer Nature. https://doi.org/10.1007/978-3-319-13521-2_23
Sunna, Anwar ; Care, Andrew ; Bergquist, PL. / Solid-binding peptides : immobilisation strategies for extremophile biocatalysis in biotechnology. Biotechnology of Extremophiles: Advances and challenges. editor / Pabulo H. Rampelotto. Cham, Switzerland : Springer, Springer Nature, 2016. pp. 637-674 (Grand Challenges in Biology and Biotechnology).
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Sunna, A, Care, A & Bergquist, PL 2016, Solid-binding peptides: immobilisation strategies for extremophile biocatalysis in biotechnology. in PH Rampelotto (ed.), Biotechnology of Extremophiles: Advances and challenges. Grand Challenges in Biology and Biotechnology, vol. 1, Springer, Springer Nature, Cham, Switzerland, pp. 637-674. https://doi.org/10.1007/978-3-319-13521-2_23

Solid-binding peptides : immobilisation strategies for extremophile biocatalysis in biotechnology. / Sunna, Anwar; Care, Andrew; Bergquist, PL.

Biotechnology of Extremophiles: Advances and challenges. ed. / Pabulo H. Rampelotto. Cham, Switzerland : Springer, Springer Nature, 2016. p. 637-674 (Grand Challenges in Biology and Biotechnology; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

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Sunna A, Care A, Bergquist PL. Solid-binding peptides: immobilisation strategies for extremophile biocatalysis in biotechnology. In Rampelotto PH, editor, Biotechnology of Extremophiles: Advances and challenges. Cham, Switzerland: Springer, Springer Nature. 2016. p. 637-674. (Grand Challenges in Biology and Biotechnology). https://doi.org/10.1007/978-3-319-13521-2_23