Some nucleotide sequences from partially purified transfer ribonucleic acids

P. L. Bergquist*, J. F. Scott

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. Transfer RNA from baker's yeast and from Escherichia coli have been fractionated by partition and ion-exchange chromatography. 2. 2. Partially purified samples of transfer RNA from yeast specific for serine and valine and from E. coli specific for phenylalanine and lysine, as well as unfractionated transfer RNA from both sources have been subjected to digestion with alkali, pancreatic RNAase (EC 2.7.7.16), and takadiastase RNAase, T1 (EC 3.1.4.8). The products of the digestions were separated by paper ionophoresis and chromatography in a two-dimensional system and identified. 3. 3. It was found that: (a) The nucleotide sequences of unfractionated transfer RNA's are non-random. (b) It appears unlikely that methylated purines occur in the serine transfer RNA reported on, and none have been identified in the partially purified samples from E. coli. (c) In transfer RNA from E. coli no runs of adenylic acid longer than 3 residues were found. (d) The following provision list of nucleotide sequences occurring in pancreatic ribonuclease digest of serine transfer RNA from yeast has been deduced: AC, 1; AU, 2; GC, 4; GU, 2; A2C, 1; A2U, 1; G2C, 2; G2U, 1; G2ψ, 1; [AG]C, 1; [AG]U, 2; [AG2]U, 2; C, 11; U, 10; and ψ, 1.

Original languageEnglish
Pages (from-to)199-211
Number of pages13
JournalBBA Specialized Section on Nucleic Acids and Related Subjects
Volume87
Issue number2
Publication statusPublished - 22 Jun 1964
Externally publishedYes

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