TY - JOUR
T1 - SOX2 protein transduction directly converts human fibroblasts into oligodendrocyte-like cells
AU - Pouya, Alireza
AU - Rassouli, Hassan
AU - Rezaei-Larijani, Mehran
AU - Salekdeh, Ghasem Hosseini
AU - Baharvand, Hossein
PY - 2020/4/23
Y1 - 2020/4/23
N2 - Oligodendrocyte precursor cells (OPCs) are ideal therapeutic cells for treatment of spinal cord injuries and diseases that affect myelin. However, it is necessary to generate a cell population with a low risk of teratoma formation and oncogenesis from a patient's somatic cells. In this study, we investigated the direct reprogramming of fibroblasts to oligodendrocyte-like cells in one step with a safe non-genetic delivery method that used protein transduction. Cell morphology and the lineage-specific marker expression profile indicated that human foreskin fibroblasts (HFFs) were converted into oligodendrocyte-like cells by the application of pluripotency factors and the use of a permissible induction medium. Our data demonstrated that SOX2 was sufficient to directly drive OPC fate conversion from HFF by a genetic-free approach. Therefore, this work has provided a strategy to OPC reprogramming by a non-integrating approach for future use in disease modeling and may ultimately provide applications for patient-specific cell-based regenerative medicine.
AB - Oligodendrocyte precursor cells (OPCs) are ideal therapeutic cells for treatment of spinal cord injuries and diseases that affect myelin. However, it is necessary to generate a cell population with a low risk of teratoma formation and oncogenesis from a patient's somatic cells. In this study, we investigated the direct reprogramming of fibroblasts to oligodendrocyte-like cells in one step with a safe non-genetic delivery method that used protein transduction. Cell morphology and the lineage-specific marker expression profile indicated that human foreskin fibroblasts (HFFs) were converted into oligodendrocyte-like cells by the application of pluripotency factors and the use of a permissible induction medium. Our data demonstrated that SOX2 was sufficient to directly drive OPC fate conversion from HFF by a genetic-free approach. Therefore, this work has provided a strategy to OPC reprogramming by a non-integrating approach for future use in disease modeling and may ultimately provide applications for patient-specific cell-based regenerative medicine.
KW - Fibroblast
KW - Oligodendrocyte
KW - Protein transduction
KW - Transdifferentiation
UR - http://www.scopus.com/inward/record.url?scp=85079363092&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2020.02.047
DO - 10.1016/j.bbrc.2020.02.047
M3 - Article
C2 - 32070492
AN - SCOPUS:85079363092
VL - 525
SP - 1
EP - 7
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -