SOX2 protein transduction directly converts human fibroblasts into oligodendrocyte-like cells

Alireza Pouya, Hassan Rassouli, Mehran Rezaei-Larijani, Ghasem Hosseini Salekdeh, Hossein Baharvand*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Oligodendrocyte precursor cells (OPCs) are ideal therapeutic cells for treatment of spinal cord injuries and diseases that affect myelin. However, it is necessary to generate a cell population with a low risk of teratoma formation and oncogenesis from a patient's somatic cells. In this study, we investigated the direct reprogramming of fibroblasts to oligodendrocyte-like cells in one step with a safe non-genetic delivery method that used protein transduction. Cell morphology and the lineage-specific marker expression profile indicated that human foreskin fibroblasts (HFFs) were converted into oligodendrocyte-like cells by the application of pluripotency factors and the use of a permissible induction medium. Our data demonstrated that SOX2 was sufficient to directly drive OPC fate conversion from HFF by a genetic-free approach. Therefore, this work has provided a strategy to OPC reprogramming by a non-integrating approach for future use in disease modeling and may ultimately provide applications for patient-specific cell-based regenerative medicine.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume525
Issue number1
DOIs
Publication statusPublished - 23 Apr 2020
Externally publishedYes

Keywords

  • Fibroblast
  • Oligodendrocyte
  • Protein transduction
  • Transdifferentiation

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