Spatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system

Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys*, Wei Deng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.

Original languageEnglish
Pages (from-to)52433-52444
Number of pages12
JournalACS Applied Materials and Interfaces
Volume12
Issue number47
DOIs
Publication statusPublished - 25 Nov 2020

Keywords

  • CRISPR-Cas9
  • light triggering
  • liposomes
  • protein release
  • temporal control

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