Spatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system

Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys*, Wei Deng

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    45 Citations (Scopus)

    Abstract

    The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.

    Original languageEnglish
    Pages (from-to)52433-52444
    Number of pages12
    JournalACS Applied Materials and Interfaces
    Volume12
    Issue number47
    DOIs
    Publication statusPublished - 25 Nov 2020

    Keywords

    • CRISPR-Cas9
    • light triggering
    • liposomes
    • protein release
    • temporal control

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