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Abstract
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
Original language | English |
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Pages (from-to) | 52433-52444 |
Number of pages | 12 |
Journal | ACS Applied Materials and Interfaces |
Volume | 12 |
Issue number | 47 |
DOIs | |
Publication status | Published - 25 Nov 2020 |
Keywords
- CRISPR-Cas9
- light triggering
- liposomes
- protein release
- temporal control
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Can we wind back the clock in adult stem cells?
Goldys, E. & Gronthos, S.
1/01/17 → …
Project: Research