Spectrophotometric and high-performance chromatographic assays of hydroperoxides by the iodometric technique

Janusz M. Gebicki; Jennifer Guille

doi:10.1016/0003-2697(89)90323-0

Spectrophotometric and high-performance chromatographic assays of hydroperoxides by the iodometric technique

Janusz M. Gebicki^*, Jennifer Guille

^*Corresponding author for this work

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I^- were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I^- allowed reading of absorbance of the I₃^- produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 10⁴ 1 mol^-1 cm^-1. Sensitivity of the assay could be improved by injecting the I₃^- into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.

Original language	English
Pages (from-to)	360-364
Number of pages	5
Journal	Analytical Biochemistry
Volume	176
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(89)90323-0
Publication status	Published - 1 Feb 1989

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title = "Spectrophotometric and high-performance chromatographic assays of hydroperoxides by the iodometric technique",

abstract = "Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I- were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I- allowed reading of absorbance of the I3- produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 104 1 mol-1 cm-1. Sensitivity of the assay could be improved by injecting the I3- into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.",

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AU - Guille, Jennifer

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N2 - Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I- were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I- allowed reading of absorbance of the I3- produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 104 1 mol-1 cm-1. Sensitivity of the assay could be improved by injecting the I3- into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.

AB - Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I- were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I- allowed reading of absorbance of the I3- produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 104 1 mol-1 cm-1. Sensitivity of the assay could be improved by injecting the I3- into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.

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