Spectrophotometric and high-performance chromatographic assays of hydroperoxides by the iodometric technique

Janusz M. Gebicki*, Jennifer Guille

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    47 Citations (Scopus)

    Abstract

    Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I- were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I- allowed reading of absorbance of the I3- produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 104 1 mol-1 cm-1. Sensitivity of the assay could be improved by injecting the I3- into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.

    Original languageEnglish
    Pages (from-to)360-364
    Number of pages5
    JournalAnalytical Biochemistry
    Volume176
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 1989

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